Fluorescent flipper probes have been introduced recently to image membrane tension in live cells, and strategies to target these probes to specific membranes are emerging. In this context, early endosome (EE) targeting without the use of protein engineering is especially appealing because it translates...
Flipper-TR ® 是一个活细胞荧光膜张力探针,实时地测量活细胞膜张力,Flipper-TR® fluorescent cell membrane tension probe 详细介绍 Flipper-TR细胞膜膜张力探针 Flipper-TR ® 是一个活细胞荧光膜张力探针,实时地测量活细胞膜张力 Flipper-TR® fluorescent cell membrane tension probe Flipper-TR®膜张力...
Cells and organelles are delimited by lipid bilayers in which high deformability is essential to many cell processes, includingmotility, endocytosis and cell division. Membrane tension is therefore a major regulator of the cell processes that remodel
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures Visualising certain organelles and their dynamics is challenging in living cells. Here the authors co-opt selective degradation to label membrane-bound compartments in worm embryos and mammalian...
Firstly, because the fluorescent probe is bound to the base of the ATP and not to the phosphate groups, one has to remember that it allows tracking nucleotide exchange on actin, but not ATP hydrolysis within actin filaments. Secondly, the presence of the fluorophore has a small impact on ...
A fluorescent membrane tension probe. Nat. Chem. https://doi.org/10.1038/s41557-018-0127-3 (2018).A. Colom, E. Derivery, S. Soleimanpour, C. Tomba, M. Dal Molin, N. Sakai, M. Gonzalez-Gaitan, S. Matile, A. Roux, `A Fluorescent Membrane Tension Probe', submitted....
membrane tensionTo image the membrane tension in living cells, planarizable push–pull probes have been introduced. The first operational probe is built around two dithieno[3,2‐b:2′,3′‐d]thiophenes (DTTs) that are twisted out of co‐planarity and polarized with donors and acceptors at ...
membrane tensionTo image the membrane tension in living cells, planarizable push-pull probes have been introduced. The first operational probe is built around two dithieno[3,2-b:2',3'-d]thiophenes (DTTs) that are twisted out of co-planarity and polarized with donors and acceptors at either...
HCl vapor exposure (Supplementary Fig.S4). In addition, fatal hydrofluoric acid (HF) vapor could be also detected using the same FSN sensor (Supplementary Fig.S5). Besides acid vapors, the sphere of sensing applications can be expanded by introducing proper probe dyes into FSN platforms40,41....
The fluorogen-activating protein biosensor design overcomes the fluorogenic probe’s limitations because each molecule of biosensor is dynamically exchanged at the plasma membrane of the cell (T1/2 = 82.5 secs; see Fig. 3), allowing for high spatial and temporal resolution of MMP14 specific ...