Fluorescent excitation and emission spectra of fluorescein isothiocyanate.Dan A. BuzatuTed J. MoskalAnna J. WilliamsWillie Mae CooperWilliam B. MattesJon G. Wilkes
Mechanism – Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications; labels do not impact hybridization performance /All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra....
The concentration of FITC-dextran is determined with a fluorescence spectrophotometer (Synergy HT, Bio-Tek Instruments, Winooski, VT) at an excitation wavelength of 480 nm and an emission wavelength of 520 nm. 13. Standard curves to calculate FITC-dextran concentration in the plasma sampl...
There is a big difference in flow cytometry although it is a small difference in the properties of these isomers in terms of excitation and emission spectra. The small difference will make your assay complicated because the ratio of two isomers varies lot by lot. BroadPharm fluorescein labeling ...
Excitation/Emission494/518 nm Product LineInvitrogen™ Unit SizeEach Contents & Storage Store in freezer (-5 to -30°C) and protect from light. Fluorescence spectra Open in SpectraViewer Documents & Downloads Certificates Search Lot #Certificate TypeDateCatalog Number(s) ...
In FIL1, the absorption and excitation spectra are both characteristic of the monoanion. In both FILs, emission of the dianion is observed upon excitation of the monoanion. This unusual behavior is interpreted in the context of a fast deprotonation of the monoanion in the excited state. The ...
Figure 1.5.3 Comparison of pH-dependent fluorescence of the Oregon Green 488 (), carboxyfluorescein () and Alexa Fluor 488 () fluorophores. Fluorescence intensities were measured for equal concentrations of the three dyes using excitatio...
This system is one of the most complex solved to date, due to the strong overlap of the absorption and emission spectra of the neutral and monoanionic forms of OG488. We also found that the cation behaves as "super" photoacid, showing a very high deprotonation rate constant (1.04 × ...
*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris...
Fig. 2.Excitation (dashed) and emission (solid) spectra of green fluorophores.Top:Fluorescein isothiocyanate (FITC)-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA). The excitation spectrum was scanned while measuring emission at 570nm, and the emission spectrum ...