FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相同时,则两FRT位点之间的基因则被删除;FRT方向相反,则基因被倒转。FRT:short flippase recognition target (FRT)。 Red recombination system中的质粒pCP20(42°培养可消除)就用于表达FLP重组酶识别FRT消除抗性。
FLP/FRT Systemdoi:10.1007/3-540-30683-8_598FLP/FRT system is a site specific recombination system found in yeast. FLP is the site-specific recombinase and the FRT is the target recognition site. The system has been adapted for use in the ...Springer Berlin Heidelberg...
Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes ZHAO Ai-Chun, LONG Ding-Pei, TAN Bing, XU Long-Xia, XIANG Zhong-Huai PDF 6125 被引次数 17 摘要/Abstract 摘要:来源于酵母2 μm质粒的FLP/FRT位点特异性重组系统已经被广泛应用于拟南芥、水稻、小鼠、果蝇、线虫...
(C) Brief scheme of knocking in vgb and marker recycling by FLP/FRT system. Note that basic principle of knocking in a heterogenous gene is the same with deletion a gene like amyL, except for carrying a heterogenous gene between a homology arm and the same side FRT. 图选项 任意挑选1株...
1) FLP/frt system FLP/frt系统 2) flp-FRT recombination system flp-FRT重组系统 3) FLP-FRT site-specific recombination FLP-FRT位点特异性DNA重组 4) FRT-relation FRT关系 1. The connections between theFRT-relationand the compatibility condition are discussed. ...
2) FLP/frt system FLP/frt系统 3) FLP-FRT site-specific recombination FLP-FRT位点特异性DNA重组 4) FLP recombinase FLP重组酶 1. The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal loci viaFLP recombinasemediated targeted integration. ...
amazonensis, and we obtained a PDC mutant without resistant marker gene successfully through this system. These research results lay a good foundation for further metabolic engineering of C. amazonensis. Key words: Candida amazonensis FLP/FRT inducible promoters gene disruption Candida amazonensis是...
Flp/FRT is a system analogous to the cre/loxP system. Flp is a yeast enzyme which recognizes FRT sites. If two FRT sites have a parallel orientation, the DNA segement between these sites will be deleted by the action of the Flp recombinase. Transgenic Animal Models...
5) site-specific recombination system 位点特异性重组系统 1. Construction of FLP/frtsite-specific recombination systemfor monocotyledonous plants; 单子叶植物的FLP/frt位点特异性重组系统的构建 更多例句>> 6) site-specific recombination 位点特异性重组技术 ...
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