(1994) Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry. 33(43):12746- 12751.Schlake, T. & Bode, J. Use of Mutated Flp Recognition Target (Frt) Sites for the Exchange of Expression Cassettes at Defined ...
从进化的角度上考虑,Flp/FRT系统是Cre/loxP系统在真核细胞内的同源系统。其中.重组酶Flp是酵母细胞内的一个由423个氨基酸组成的单体蛋白。与Cre相似,Flp发挥作用也不需要任何辅助因子,同时在不同的条件下具有良好的稳定性。该系统的另一个成分Flp识别位点(Flp recognition target,FRT)与loxP位点非常相似,同样由两个...
摘要: Examines the use of the FLP Recognition Target (FRT) system for site-specific recombination and the wild-type recognition site in conjunction with certain mutant FRT sites in provoking an exchange of expression cassettes at defined chromosomal loci. Design of mutant FRT sites....
如果Arg308 发生突变,则会影响FLP 单体对 作用下,2 个相同的间隔区突变型FRT 位点(如FRT3 3254 中国农业科学 44 卷 表 可供选择的FLP识别位点 Table Alternative FLP recognition sites 靶位点 反向重复序列1 间隔区域 反向重复序列2 供选择位点的用途 参考文献 Target site a Inverted repeat 1 Spacer Inverted...
The 21-kDa peptide contains a site-specific DNA-binding domain that binds to the FLP recognition target (FRT) site with an affinity similar to that observed for the native FLP protein. This peptide can induce DNA bending upon binding to a DNA fragment containing the FRT site, but the angle...
如果Arg308 发生突变,则会影响FLP 单体对 作用下,2 个相同的间隔区突变型FRT 位点(如FRT3 3254 中国农业科学 44 卷 表 可供选择的FLP识别位点 Table Alternative FLP recognition sites 靶位点 反向重复序列1 间隔区域 反向重复序列2 供选择位点的用途 参考文献 Target site a Inverted repeat 1 Spacer Inverted...
FLP recombinase induces a double-reciprocal crossover event between sets of different FLP recognition target (FRT) sites. Therefore, if these sites flank an expression cassette at a given genomic locus, it can be exchanged for another cassette that has been constructed in the analogous way [Schla...
We describe here a highly efficient procedure for conditional mutagenesis in Plasmodium. The procedure uses the site-specific recombination FLP-FRT system of yeast and targets the pre-erythrocytic stages of the rodent Plasmodium parasite P. berghei, incl
They cleave and covalently attach to their target sites (for example, the Flp recognition target, FRT site, see Fig. 1a) and they are able to recombine DNA with exquisite fidelity and very high efficiency. Figure 1 a). The structure of the FRT site. The FRT site consists of three 13 ...
Depending on the location and direction of the flippase recombination target (FRT) recognition sites, FLP recombinase can invert, recombine, and position the DNA sequence between the recognition sites [5]. When FRT recognition sites have different orientations and are located on the same chromosome,...