The PCR product was cloned to a BglII site upstream of the flippase recognition target (FRT) in pKD13 (20). Expansion of Shiga toxin-producing Escherichia coli by use of bovine antibiotic growth promoters ATP8B1 encodes a P-type ATPase, FIC1, which is an aminophospholipid flippase that flip...
Objectives To establish a recombinase flippase (FLP) and flippase recognition target (FRT) system-mediated protocol for post-integration excision of exogenous DNA fragments in the oleaginous yeast Rhodosporidium toruloides.Sun, WenyiYang, XiaobingWang, XueyingJiao, XiangZhang, SufangLuan, YushiZhao, ...
Penicillium oxalicum has received increasing attention as a potential cellulase-producer. In this study, a copper-controlled flippase recombination enzyme/recognition target (FLP/FRT)-mediated recombination system was constructed in P. oxalicum, to overcome limited availability of antibiotic resistance ...