Flow field-flow fractionation (FlFFFProtein separationProteomicsProteome analysis requires a comprehensive approach including high-performance separation methods, mass spectrometric analysis, and bioinformatics. While recent advances in mass spectrometry (MS) have led to remarkable improvements in the ability ...
Asymmetrical flow field-flow fractionation (AF4) is a well-established and highly versatile technique for sizing and quantifying aggregates and particles in protein formulations. AF4 separates the various species in a channel consisting of a non-permeable plexiglass upper wall and a semipermeable ac...
We describe the protocol development and optimization of asymmetric-flow field-flow fractionation (AF4) technology for separating and characterizing extracellular nanoparticles (ENPs), particularly small extracellular vesicles (sEVs), known as exosomes, and even smaller novel nanoparticles, known as exomere...
Principle: Asymmetric flow field-flow fractionation (AF4), sometimes more simply shortened field-flow fractionation (FFF), is a separation methodology that separates macromolecules and particles in solution according to differences in size. The separation is based on the macromolecule’s differential loca...
and support for investigations. The past several decades have seen demonstration and documentation of the utility of asymmetrical flow field-flow fractionation (AF4) in biotechnology applicable to each of these protein drug development phases, but as yet, with limited industrial or routine implementation...
Flow field-flow fractionation is a new separation technique that seems likely to have broad applicability. Its theoretical scope includes any solute for which one can find a solvent and a semipermeable membrane. The principles on which the technique is based are presented. Some experiments with poly...
Flow Field-Flow Fractionation 1986 Flow Field-Flow Fractionation has been the most important Field-Flow Fractionation technique for over two decades. The world’s first commercial Flow Field-Flow Fractionation instrument, the Model F1000, was invented and commercialized in 1986 by FFFractionation which...
Asymmetrical flow field-flow fractionation (AF4) is a high-resolution particle separation technique operating in one-phase chromatography. The design of channels consists of an upper wall impermeable to flow and the lower wall permeable with a typical membrane size of about 10 kDa. The AF4 operat...
An alternative separation technique, asymmetrical flow field-flow fractionation (AF4) has attracted more and more attention in recent years due to its wide separation range (from 1 nm to much greater than 1 μm) as well as an utilization of open separation channel (Yohannes, Jussila, Hartonen...
We present a generically applicable approach to determine an extensive set of size-dependent critical quality attributes inside nanoparticulate pharmaceutical products. By coupling asymmetrical-flow field-flow fractionation (AF4) measurements directly in