Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the ...
Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as serum and saliva, including the assessment of activity of possibly aggregated species. The coupling of...
Principle: Asymmetric flow field-flow fractionation (AF4), sometimes more simply shortened field-flow fractionation (FFF), is a separation methodology that separates macromolecules and particles in solution according to differences in size. The separation is based on the macromolecule’s differential loca...
We describe the protocol development and optimization of asymmetric-flow field-flow fractionation (AF4) technology for separating and characterizing extracellular nanoparticles (ENPs), particularly small extracellular vesicles (sEVs), known as exosomes, and even smaller novel nanoparticles, known as exomere...
Asymmetric flow field-flow fractionation (AF4) coupled with dynamic light scattering or multiangle light scattering detectors is a promising technique for the size-based separation of colloidal particles (nano- and submicron scale) and the online determination of the particle size of the separated fra...
In this work, sample losses of silver nanoparticles (Ag NPs) in asymmetrical flow field-flow fractionation (AF4) have been systematically investigated with the main focus on instrumental conditions like focusing and cross-flow parameters as well as sample concentration and buffer composition. Special ...
Casein micelles Skim milk powder Asymmetric flow field-flow fractionation AF4 Powder reconstitution Dairy technology 1. Introduction Milk powders are commonly used in the food industry as they have an extended shelf-life and facilitate transportation (Martin, Williams, & Dunstan, 2007). As a result...
C. Zielke, C. Teixeira, H. Ding, S. Cui, M. Nyman, L. Nilsson, Analysis of beta- glucan molar mass from barley malt and brewer's spent grain with asym- metric flow field-flow fractionation (AF4) and their association to proteins, Carbohydr. Polym. 157 (2017) 541e549....
In this short communication we report on the technical implementations of coupling an asymmetric flow field-flow fractionation (AF4) instrument to a high r... P Herrero,PS B?Uerlein,E Emke,... - 《Journal of Chromatography A》 被引量: 27发表: 2014年 Asymmetric Flow-Field Flow Fractionatio...
Stepan Podzimek: Light scattering, size exclusion chromatography and asymmetric flow field flow fractionation. Powerful tools for the characterization of p... Asymmetric flow field-flow fractionation (AF4) enables to analyse polymers with very high molar masses under mild conditions in comparison to si...