S. Podzimek, P. Lebeda, C. Johann, Asymmetric Flow Field Flow Fractionation: A Powerful Method for Polymer Characterization, LCGC The Application Notebook, 2009, pp. 62-66, Advanstar Communications, US.S. Podzimek, P. Lebeda, C. Johann, Asymmetric Flow Field Flow Fractionation: A Powerful...
Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as serum and saliva, including the assessment of activity of possibly aggregated species. The coupling of...
Bouby, M, Geckeis, H, Geyer, FW (2008) Application of asymmetric flow field-flow fractionation (AsFlFFF) coupled to inductively coupled plasma mass spectrometry (ICPMS) to the quantitative characterization of natural colloids and synthetic nanoparticles. Anal Bioanal Chem 392: pp. 1447-1457...
We describe the protocol development and optimization of asymmetric-flow field-flow fractionation (AF4) technology for separating and characterizing extracellular nanoparticles (ENPs), particularly small extracellular vesicles (sEVs), known as exosomes, and even smaller novel nanoparticles, known as exomere...
Principle: Asymmetric flow field-flow fractionation (AF4), sometimes more simply shortened field-flow fractionation (FFF), is a separation methodology that separates macromolecules and particles in solution according to differences in size. The separation is based on the macromolecule’s differential loca...
In this work, sample losses of silver nanoparticles (Ag NPs) in asymmetrical flow field-flow fractionation (AF4) have been systematically investigated with the main focus on instrumental conditions like focusing and cross-flow parameters as well as sample concentration and buffer composition. Special ...
In this study, we present the characterization and optimization of an asymmetric flow field-flow fractionation (AF4) system coupled with UV/Vis spectrophotometer and dynamic light scattering (DLS) detector as a powerful tool for the size separation and multi-parameter characterization of AgNPs in ...
Analytical Bioanalytical Chemistry Electronic Supplementary Material PEGylated gold nanorod separation based on aspect ratio: characterization by asymmetric-flow field flow fractionation with UV-Vis detection Thao M. Nguyen, Julien Gigault, Vincent A. Hackley 1. FFF methodology In ...
Casein micelles Skim milk powder Asymmetric flow field-flow fractionation AF4 Powder reconstitution Dairy technology 1. Introduction Milk powders are commonly used in the food industry as they have an extended shelf-life and facilitate transportation (Martin, Williams, & Dunstan, 2007). As a result...
Asymmetric flow field-flow fractionation (AF4) coupled with dynamic light scattering or multiangle light scattering detectors is a promising technique for the size-based separation of colloidal particles (nano- and submicron scale) and the online determination of the particle size of the separated fra...