Conditions for detecting BCR-ABL1 fusion proteins throughin situPLA with flow cytometry as readout, PLA-flow (Fig.1), were established using K562 cells that carry the BCR-ABL1 fusion, while the BCR-ABL1 negative cell line U937 was used as a negative control. Briefly, cells to be analyzed...
To that end, demonstrating the ease and expediency of developing life science and analytical instruments from the ground up, Excelitas Technologies and its subsidiary Qioptiq decided to build a fully functional modular flow cytometry prototype. This prototype uses off-the-shelf products from Excelitas’...
各种标记都被描述为特征 CD1233、CLL-14、CD975或TIM-36等。CD34+CD38 的爆破细胞的隔室被认为是丰富的, 为7 , 但也包括正常的 HSC。CD90 和 CD45RA 表达式应用于 CD34+CD38 (P6) 隔间 (图1) 允许隔离正常和恶性造血前体的几个阶段8。具体地说, 正常的 CD34+CD38-CD90+CD45RA-HSCs, 多能祖...
Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL)Flow cytometryLEF1Nuclear overexpression of lymphoid enhancer-binding factor 1 (LEF1) assessed by immunohistochemistry has been shown to be highly associated with chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) among small B...
9 Compared to histopathology and immunohisto- chemistry, flow cytometry allows for a closer examination of tumor heterogeneity and identifica- tion of subtle differences with regard to antigen expression. Also, fine needle aspiration biopsy proved to be highly efficient way of obtaining tumor cells ...
Recent advances in automation technologies have enabled the use of flow cytometry for high throughput screening, generating large complex data sets often in clinical trials or drug discovery settings. However, data management and data analysis methods have not advanced sufficiently far from the initial ...
Interpretation ZAP-70 protein, which can be measured by flow cytometry in the general laboratory, is a reliable prognostic marker in CLL, equivalent to ... Jenny A Orchard and Rachel E Ibbotson and Zadie Davis and Adrian Wiestner and Andreas Rosenwald and Peter W Thomas and Terry J Hamblin ...
Minimal or measurable residual disease (MRD) detected by multiparameter flow cytometry (MFC) is an independent prognostic indicator in acute leukemia. Howe
Systematic donor blood qualification by flow cytometry would have been able to avoid CLL-type MBL transmission after unrelated hematopoietic stem cell transplantation. Eur J Haematol. 2012;88(3):269-272.Ferrand C, Garnache-Ottou F, Collonge-Rame MA, et al. Systematic donor blood qualification ...
Although several flow cytometry assays have been described for assessing ZAP‐70 in CLL, certain technical and scientific issues remain unsolved, which have prevented results of this crucial test from being reported, even in the best routine flow cytometry laboratories. In this report, we aimed to...