[7]Rawstron AC, Kreuzer KA, Soosapilla A, et al. Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project. Cytometry B Clin Cytom. 2018 Jan;94(1):121-12...
Keywords chroniclymphocyticleukemia;CLLflowscoresystem;flowcytometry;CD200 B 细胞慢性淋巴细胞增 殖性疾病(Bcellchro- niclymphoproliferativedisorders,B-CLPD)是一 组在形态学和免疫表型特征以及临床行为上均有 异质性的疾病 .[1] 由于不同疾病类别的临床演变 和治疗反应可 能不同,因此正确的诊断至关重要. 流...
Acute myeloid leukemia (AML) that occurs concurrent with a diagnosis of chronic lymphocytic leukemia (CLL) is rare, but the number of cases recognized has recently dramatically increased as a result of the application of flow cytometry. This raises a series of questions regarding the clinical ...
A flow Cytometry test is used to specifically diagnose CLL. The FISH test would show aggressive strains of CLL. Both can be done with blood now, but when I was diagnosed (15 years ago) they were routinely done as part of a bone marrow biopsy (BMB) and still can be done that way or...
Secondary end points at 3 months post-treatment included: MRD negativity assessed in the bone marrow by highly sensitive multiparameter flow cytometry with a level of detection o 1 CLL cell in 10 000 leukocytes;13 ORR defined as at least partial remission (PR); and safety and toxicity as ...
ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis. Cytometry B Clin Cytom 2006; 70: 235–241. 22 Hamblin TJ, Davis Z, Gardiner A, Oscier DG, Stevenson FK, Unmutated Ig V(H. genes are associated with a more aggressive form of ...
The expression of CD38 and ZAP-70 was examined in a cohort of 56 patients with CLL by flow cytometry, with a cut-off positivity value of 20% and higher and 10% and higher, respectively (Table 1). The cut-off values were experimentally defined as functional thresholds (ie, values below ...
Flow cytometric visualisation of cytokine production by CD3− CD56+ NK cells and CD3+ CD56+ NK-T cells in whole blood. Cytometry 2000; 39: 72–78. Article CAS PubMed Google Scholar Rezvany MR, Jeddi-Tehrani M, Rabbani H, Ruden U, Hammarstrom L, Osterborg A . Autologous T ...
Analysis was performed at the UNMC Flow Cytometry Facility. Western Blot. Western blots were performed as previously described12 with use of the following antibodies: Dnmt3b (52A1018, Imgenex), Dnmt1 (H-300, Santa Cruz), and HDAC1 (ab7028, Abcam). Combined Bisulfite Restriction Analysis (...
MRD negative < 10−4; positive ≥ 10−4 detected CLL cells per leukocytes, according to MRD-flow cytometry. For MRD-results from bone marrow please refer to Supplementary Figs. 1 and 2. Full size image After 5 years from the last dose of study medication, 80.4% of Hi-FCR ...