[7]Rawstron AC, Kreuzer KA, Soosapilla A, et al. Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project. Cytometry B Clin Cytom. 2018 Jan;94(1):121-12...
Keywords chroniclymphocyticleukemia;CLLflowscoresystem;flowcytometry;CD200 B 细胞慢性淋巴细胞增 殖性疾病(Bcellchro- niclymphoproliferativedisorders,B-CLPD)是一 组在形态学和免疫表型特征以及临床行为上均有 异质性的疾病 .[1] 由于不同疾病类别的临床演变 和治疗反应可 能不同,因此正确的诊断至关重要. 流...
stains|Flow cytometry description|Flow cytometry images|Molecular / cytogenetics description|Molecular / cytogenetics images|Sample pathology report|Differential diagnosis|Additional references|Board review style question #1|Board review style answer #1|Board review style question #2|Board review style answer...
C Representative flow cytometry scatter plots of T cells after culture with the indicated condition. P values: * <0.05; ** <0.01; *** <0.001. Full size image Next, CD4+ CLL TN cells were stimulated in the same manner in the presence of the two MDSC subtypes. PMN-MDSCs led to a ...
8 Lymphocytes were isolated by Ficoll density-gradient centrifugation, and immunofluorescence flow cytometry verified that they were composed of greater than 90% CD5+CD19+CD23+ B cells. Cell viability and apoptosis assay CLL B cells were cultured at 2 × 106/mL in Iscove modified Dulbecco ...
The most impressive finding was the very high rate of negativity for MRD, which means that there was no detectable CLL by flow cytometry in either the blood or the bone marrow. By this traditional measure of MRD, we could not find any CLL cells—and that was in over half of pat...
Acute myeloid leukemia (AML) that occurs concurrent with a diagnosis of chronic lymphocytic leukemia (CLL) is rare, but the number of cases recognized has recently dramatically increased as a result of the application of flow cytometry. This raises a series of questions regarding the clinical ...
Secondary end points at 3 months post-treatment included: MRD negativity assessed in the bone marrow by highly sensitive multiparameter flow cytometry with a level of detection o 1 CLL cell in 10 000 leukocytes;13 ORR defined as at least partial remission (PR); and safety and toxicity as ...
Flow cytometry was performed at baseline and designated time points. After viability assessment, samples were stained using PrepPlus2 automated staining system (Beckman Coulter) using five color whole blood staining technique with panels of directly conjugated monoclonal antibodies. Following 30 minutes of...
All patients enrolled to this study had stimulated cytogenetics, FISH, and IGHV mutational status performed at baseline as previously described (Byrd et al, J Clin Oncol (2006) 24, 437-43; Woyach et al, Br J Haematol (2010) 148, 754-9. Flow cytometry was performed at baseline and desig...