Flow CytometryGatingStandardizedGating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for...
流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
Considerations for Flow Cytometry Gating There are a number of factors to consider and incorporate into your FACS staining and gating strategy, including: Compensating for spectral overlapUsing appropriate gating controlsExcluding debrisExcluding doublets or multipletsExcluding non-viable cellsStaining with a ...
To perform rare event analysis in research settings using flow cytometry, the quantity of biological material, number of events to acquire, as well as the markers that identify the population of interest should be considered...
Flow cytometry data analysisis fundamentally based upon the principle of gating. Gates and regions are placed around populations of cells with common characteristics, usually forward scatter, side scatter and marker expression, to investigate and to quantify these populations of interest. Here we will ...
What if that debris isnotdebris? One of the reasons gating is scary is that it is subjective and relies a little on your/the cytometrist’s judgment. It also forms the beginning of any cytometry experiment, in that what you get out at the end relies on what you do at this stage. ...
flow cytometry procedure (Majka et al., 2012) that effectively excludes stromal/vascular cells from adipocyte preparations. The strategy involved gating or acquisition of cells whose size and internal complexity were greater than stromal/vascular cells, exclusion of cell aggregates, and exclusion of ...
These multiparameter measurements increase assay specificity while also providing a strategy for identifying and eliminating cells from further analysis (e.g., see “Gating strategies for maximizing assay specificity” below), each of w...
Using the above gating methods, you now should be able to confidently analyze your cells and make accurate conclusions based on your flow cytometry analysis. Learn more about gating and see a full length gating example. Build multicolor flow cytometry panels in just a few simple steps ...
7. Analyze by flow cytometry using the gating strategy described for the live whole- blood procedure (Subheading 3.1.1.) or, if the erythrocytes have been lysed, by gating on the relevant leukocytes in a display of forward and side light scatter. 6.6. Determining Absolute ...