the maximum percentage of mismatched bases to detect overlapped region ofPEreads.Thiswill affect overlap analysis basedPEmerge,adapter trimming and correction.Default20means20%.(int[=20]) 本次测试的数据为PE250,共85,109对reads。 mkdir -p fastp mkdir -p SE ls *1.fastq.gz|while read id; do ...
Usage:flash[OPTIONS] MATES_1.FASTQ MATES_2.FASTQflash[OPTIONS]--interleaved-input(MATES.FASTQ|-)flash[OPTIONS]--tab-delimited-input(MATES.TAB|-)---DESCRIPTION---FLASH(FastLengthAdjustmentofSHortreads)isan accurate and fast tool to merge paired-end reads that were generated fromDNAfragments whose...
lengths OVERLAP_LEN=100 # Define read length, will use this to define the maximum overlap READ_LEN=126 # Run FLASH to merge overlapping reads flash \ R1.fastq.gz R2.fastq.gz \ --min-overlap=$OVERLAP_LEN \ --max-overlap=$READ_LEN \ --output-prefix=test \ --output-directory=results...
{node: '>= 8'} dependencies: '@nodelib/fs.scandir': 2.1.5 fastq: 1.17.1 /@nolyfill/is-core-module@1.0.39: resolution: {integrity: sha512-nn5ozdjYQpUCZlWGuxcJY/KpxkWQs4DcbMCmKojjyrYDEAGy4Ce19NN4v5MduafTwJlbKc99UA8YhSVqq9yPZA==} engines: {node: '>=12.4.0'} dev: true /@...