FASTQ filenames are often based on the sample identifier, e.g. SampleA_R1.fastq. If you specify -relabel @ then fastq_mergepairs gets the sample identifier from the FASTQ file name by truncating at the first underscore (_) or period (.). A period and the read number is added after ...
在扩增子测序的质量控制这一步骤中,在执行./usearch11 -fastq_mergepairs SH_R1.fq-relabel @ -fastq_maxdiffs 10 -fastq_pctid 80 -fastqout SH.fq之后,输出的文件为()? SH_R1.fqSH_R2.fqSH.fqSH.fa 相关知识点: 试题来源: 解析 SH.fq ...
-fastq_mergepairs Forward FASTQ filename(s). -reverse Reverse FASTQ filename(s). If not given, constructed by replacing R1 with R2. -interleaved Forward and reverse reads are interleaved in the same file (sometimes produced by SRA fastq-dump). ...
usearch -fastq_mergepairs SampleA_R1.fastq -fastqout merged.fq Merging multiple FASTQ file pairs in a single command You can specify two or more FASTQ filenames following -fastq_mergepairs. In the following example, SampleA and SampleB are both merged. The R2 filenames are constructed auto...
usearch -fastq_mergepairs *_R1_*.fastq -fastqout merged.fq -relabel @ The -fastq_merge_maxee option can be used to set anexpected errorsthreshold, but for OTU clustering quality filtering is generally performed as a post-processing step: ...