本试剂盒包含高质量的Anti-Flag琼脂糖凝胶、小鼠IgG琼脂糖凝胶(作为阴性对照)及优化的各种缓冲液如Lysis Buffer、10×TBS、100×Protease Inhibitor Cocktail、3X Flag Peptide(25×)、Acid Elution Buffer、Neutralization Buffer、5×SDS-PAGE Sample Loading Buffer等免疫沉淀必要试剂,使免疫沉淀或免疫共沉淀实验更加简单...
One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first ...
FLAG peptide | MedChemExpress 英文名称: 总访问: 142 国产/进口: 进口 半年访问: 11 产地/品牌: Medchemexpress (MCE) 产品类别: 生化试剂 规格: 1 mg; 5 mg; 10 mg; 25 mg 最后更新: 2024-11-4 货号: HY-P0223 CAS 号: 98849-88-8 参考报价: 500; 900; 1300; 2390 立即询价 电话咨询 ...
本试剂盒包含高质量的BalbMag Anti-Flag磁珠、BalbMag小鼠IgG磁珠(作为阴性对照)及优化的各种缓冲液如Lysis Buffer、10×TBS、100×Protease Inhibitor Cocktail、3X Flag Peptide(25×)、Acid Elution Buffer、Neutralization Buffer、5×SDS-PAGE Sample Loading Buffer等免疫沉淀必要试剂,使免疫沉淀或免疫共沉淀实验更加...
Peptide Elution of 3X FLAG ® Fusion Protein from ANTI-FLAG ® M2 Affinity Gel Note: Affinity chromatography may be performed at room temperature. If, however, the 3X FLAG ® fusion protein is unstable or sensitive to protease,chromatography should be performed at 2-8 °C. Column Set-Up...
FLAG peptide allows elution under non-deturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with...
flag填料的elution
Another strategy is competitive elution with excess of free FLAG™ peptide. Antibodies M2 and M5 are applied in this procedure. Examples demonstrating the versatility, practicability and limitations of this technology are given.A.Einhauerand
本试剂盒包含高质量的Anti-Flag Agarose Gel (Anti-Flag琼脂糖凝胶)、Mouse IgG Agarose Gel (小鼠IgG琼脂糖凝胶,作为阴性对照)及优化的各种缓冲液如Lysis Buffer、TBS (10X)、Protease Inhibitor Cocktail (100X)、3X Flag Peptide (25X)、Acid Elution Buffer、Neutralization Buffer、SDS-PAGE Sample Loading Buffe...
4.3 柔和洗脱方式(flag peptide 和 TBS 未提供): A.准备 200ug-1mg/mL Flag Peptide 的 flag peptide 溶解在 TBS 中. B.向珠子中加入 100 微升 200ug-1mg/mL Flag Peptide,轻轻涡旋混合,并在旋转器上于 4℃孵育样品 2-6 小时. C.在磁力架上分离磁珠,并保存含有目标抗原的上清液. D.重复一次洗脱步骤...