本试剂盒包含高质量的Anti-Flag琼脂糖凝胶、小鼠IgG琼脂糖凝胶(作为阴性对照)及优化的各种缓冲液如Lysis Buffer、10×TBS、100×Protease Inhibitor Cocktail、3X Flag Peptide(25×)、Acid Elution Buffer、Neutralization Buffer、5×SDS-PAGE Sample Loading Buffer等免疫沉淀必要试剂,使免疫沉淀或免疫共沉淀实验更加简单...
One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first ...
flag填料的elution
FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution...
FLAG peptide allows elution under non-deturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with...
Peptide Elution of 3X FLAG ® Fusion Protein from ANTI-FLAG ® M2 Affinity Gel Note: Affinity chromatography may be performed at room temperature. If, however, the 3X FLAG ® fusion protein is unstable or sensitive to protease,chromatography should be performed at 2-8 °C. Column Set-Up...
It allows elution under nondenaturing conditions. Several antibodies against this peptide have been developed. Oneantibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitiveelution with ...
Another strategy is competitive elution with excess of free FLAG™ peptide. Antibodies M2 and M5 are applied in this procedure. Examples demonstrating the versatility, practicability and limitations of this technology are given.A.Einhauerand
本试剂盒包含高质量的Anti-Flag Agarose Gel (Anti-Flag琼脂糖凝胶)、Mouse IgG Agarose Gel (小鼠IgG琼脂糖凝胶,作为阴性对照)及优化的各种缓冲液如Lysis Buffer、TBS (10X)、Protease Inhibitor Cocktail (100X)、3X Flag Peptide (25X)、Acid Elution Buffer、Neutralization Buffer、SDS-PAGE Sample Loading Buffe...
Storage Temperature −20 °C Product Description Monoclonal ANTI-FLAG ® M2 is a mouse-derived,affinity-purified IgG1 monoclonal antibody that binds to fusion proteins containing a FLAG ® peptide sequence.1 The M2 antibody will recognize a FLAG ® peptide sequence at the N-terminus,Met-...