FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for sensitive immunoprecipitation and FLAG peptides permit efficient elution of protein complexes. With many commercially available FLAG tools, this protocol is highly versatile. This procedure reduces immunoprecipitation of ...
Product Description Monoclonal ANTI-FLAG ® M2 is a mouse-derived,affinity-purified IgG1 monoclonal antibody that binds to fusion proteins containing a FLAG ® peptide sequence.1 The M2 antibody will recognize a FLAG ® peptide sequence at the N-terminus,Met-N-terminus, C-terminus, or ...
工作液配置:3xFLAG peptide常用工作液浓度为100ug/ml,可用于从Anti-FLAG M2亲和凝胶上洗脱3xFLAG融合蛋白。5倍柱体积的工作溶液即可充分洗脱大部分3xFLAG融合蛋白 二:FLAG-Tag Protein Purification Protocol for Mammalian Cells General Notes The following protocol is based on and optimized for over expressed FLA...
PeptideStructure Molecular Formula C120H169N31O49S Molecular Weight 2861.9 订购联系方式: 上海鸿肽生物科技有限公司 手机:+86-18018095145(同微信号) QQ: 3259098395 邮箱:sales1@hongtide.com 关键字:402750-12-3;3xflag ;多肽标签3X FLAG;3X FLAG Peptide;多肽; ...
The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts. Anti-FLAG® M2 Magnetic Beads Comparison of elution techniques for small-scale protein purification...
Mild elution conditions are generally a NaCl gradient at alkaline pH. This tag also binds to flat mica sheets, which may enable a variety of new applications. Antibody–Epitope Interaction Affinity Tags Recombinant DNA technology has been employed to create peptide epitopes that bind well-...
up to 10 ml or larger)Transfer and Wash Resin (Transfer resin to a Spin column and wash)st Elution of FLAG-HA-tagged Protein (Competitive elution with 3x FLAG peptide)Bind to Anti-HA resin (Incubate in a Spin column)Wash Resin nd Elution of Protein/protein Complex downstream applications)
? Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide). Other Notes Kit contains CelLytic? M for cell lysis and protein extraction from Mammalian cells. 包装 Sufficient for 3-5 uses of 1-ml affinity purification column. ...
A. Neutral Elution Protocol 1. Prepare DYKDDDDK peptide (PP101274) at 1mg/mL in PBS. 2. Add 50 μL 1 mg/mL DYKDDDDK peptide to the Immunomagnetic Beads, gently vortex to mix and incubate the sample at 37 ℃ on a rotator for 5-10 min. Elution may be performed at reduced temperatures...
Anti-FLAG (DYKDDDDK) Tag agarose Beads were used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Peptide elution buffer, the enriched FLAG fusion protein are released from the agarose beads.