Wandless, T J
Label-free Single-Molecule Quantification of Rapamycin-induced FKBP–FRB Dimerization for Direct Control of Cellular Mechanotran Subscriber access provided by Macquarie Universityis published by the American Chemical Society. 1155 Sixteenth Street N.W.,Washington, DC 20036Published by American Chemical Socie...
research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
Chaurasia, S., Pieraccini, S., De Gonda, R., Conti, S., & Sironi, M. (2013). Molecular insights into the stabilization of pro- tein-protein interactions with small molecule: The FKBP12-rapamycin-FRB case study. Chemical Physics Letters, 587, 68-74. doi:10.1016/j.cplett.2013.09.042...
We previously advanced an inducible dimerization system into an inducible oligomerization system by developing a bivalent fusion protein, FRB–FKBP, which forms large oligomers upon rapamycin addition and can be used to manipulate cells. However, the oligomeric structure of FRB–FKBP remains unclear. ...
To probe the affinities involved in the formation of the FKBP-rapamycin-FRB complex, we used fluorescence polarization, surface plasmon resonance, and NMR spectroscopy. Analysis of the data shows that rapamycin binds to FRB with moderate affinity (Kd =26 ± 0.8 μM). The FKBP12·rapamycin ...
The present study exemplifies the PPI in vitro inside a unique single-chain probe, named TP2.4, which carries a full-length artificial luciferase (ALuc) sandwiched in between two model proteins of interest, e.g., FKBP and FRB, expressed in E. coli, and purified. We found that the TP2.4...
FRBFKBPRapamycinOligomerChemically-induced oligomerizationProtein engineeringMost proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls the cellular functions that involve protein complexes. Although several artificial ...
Laura A.Banaszynski,Corey W.Liu,Thomas J.Wandless. Characterization of the FKBP-Rapamycin-FRB Ternary Complex[J].Journal of the American Chemical Society 2005,13(13).Banaszynski LA , Liu CW , Wandless TJ . 2006b . Characterization of the FKBP–rapamycin–FRB ternary complex . J Am C...
Here, we engineered a rapamycin-induced dimerization system of Fas consisting of FKBP and FRB proteins. Treatment of rapamycin specifically induces cellular apoptosis. In neurons and cells with high c-FLIP expression, rapamycin-induced Fas activation triggered the activation of the non-apoptotic pathway...