Further test using immuno-gold labeling with anti-keratin antibodies indicated that the component of the intermediate filament-like system was keratin-like protein. Such a keratin-like intermediate filament system existing in plant cells was demonstrated for the first time. Meanwhile, 3-nm size ...
DNA binding assays established that MFP1 can discriminate between animal and plant MAR DNAs and non-MAR DNA fragments of similar size and AT content. Deletion mutants of MFP1 revealed a novel, discrete DNA binding domain near the C terminus of the protein. MFP1 is an in vitro substrate ...
Coiled-coil domains of intermediate filaments (IF) and prokaryotic IF-like proteins enable oligomerisation and filamentation, and no additional function is ascribed to these coiled-coil domains. However, an IF-like protein fromStreptomyces reticuliwas reported to display cellulose affinity. We demonstrat...
The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts, which was identified by using immunogold labelling with monoclonal antibody of cytokeratin from animal cells. Many gold particles were...
Therein, myosin, actin, and collagen have been selected to be the representative members in natural protein filaments. Then the synthesized protein filaments have been divided into animal-sourced proteins, plant-sourced proteins, and microbial-sourced proteins. We concluded by highlighting the current ...
1.a very fine thread or threadlike structure; a fiber or fibril:filaments of gold. 2.the stalklike portion of a stamen, supporting the anther. 3.(in an incandescent lamp) the threadlike conductor, often of tungsten, that is heated to incandescence by the passage of current. ...
Natural fibres(Table 1.1) are any fibres that exist as fibres in their natural state. They can be categorised as protein (animal),cellulosic(vegetable) and mineral. Protein fibres are sheep’s wool, speciality wools and silk. Speciality wools include goats’ wool such as cashmere and mohair ...
plant cells. The protein encoded by AT3G05270 has a large α-helix as well as the IF protein motif indispensable for maintaining the structures of IF. Moreover, fluorescence signals of this protein fused with GFP exhibited cytoskeleton-like filamentous structures in plant cells. Thus, we named...
we turned toAgrobacterium tumefaciens-mediated transformation (AtMT), an insertional mutagenesis approach with a history of proven success in fungal species22.A. tumefaciensis a plant pathogen that causes crown gall in dicotyledonous plants through genetic transformation23. Its capacity for transformation ...
Auxin regulates plant growth and development in part by activating gene expression. Arabidopsis thaliana SMALL AUXIN UP RNAs (SAURs) are a family of early auxin-responsive genes with unknown functionality. Here, we show that transgenic plant lines expressing artificial microRNA constructs (aMIR-SAUR-...