Fixed internal diaphragms of FN=16 mm and FN=20 mm eyepieces. 1. Eyepiece diaphragm The microscope field of view diameter in the plane where the specimen is placed is defined by the following formula:where DFV is the diameter of the view field in the specimen plane, ...
W B Dou,X D Deng,J H Pan.ANALYSIS OF EXTENDING THE FIELD-OF-VIEW OF REVERSE-MICROSCOPE IMAGING SYSTEM AT MILLIMETER-WAVELENGTHS[J]. Journal of Electromagnetic Waves and Applications .2004(4)W.B Dou, X.D. Deng.Analysis of Expending the field-of-view of Reverse-Microscope imaging System ...
For microscopy imaging, depth of field is an important parameter when needing sharp images of sample areas with structures having significant changes in depth. In practice, depth of field is determined by the correlation between numerical aperture, resol
In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function of the brain's circuitry. Multiphoton microscopy, a powerful tool for deep-tissue imaging, has received sustained interest in advancing its speed, field of view and imaging depth. However, to avoid ...
by mapping the low-resolution multi-view data to high-resolution 3D volumes directly, the spatial resolution of current learning-based LFM is far from sufficient for subcellular structures, due to the huge resolution gap between the raw light-field measurements and the diffraction limit of the obje...
Here, we incorporate a two-stage view-channel-depth (VCD) deep-learning reconstruction strategy with a Fourier light-field microscope based on diffractive optical element to realize fast 3D super-resolution reconstructions of intracellular dynamics from single diffraction-limited 2D light-filed ...
For instance, in electron microscope tomography (EMT), the configurations of the sample and electron beam limit the range of angles for which projections (i.e., the sinogram) are acquired. This creates reconstruction artifacts that adversely affect the quality of virtually any direct rendering or ...
View chapter Book series 2001, Studies in Surface Science and CatalysisM. Futamata, ... M. Ishikawa Chapter Scanning Probe Microscopy, Theory The Scanning Near-Field Optical Microscope-Optical Spectroscopy The theory of scanning near-field optical microscopy is somewhat similar to that of STM, with...
X-ray spectromicroscopy with a full-field imaging technique is a powerful method for chemical analysis of heterogeneous complex materials with a nano-scale spatial resolution. For imaging optics, an X-ray reflective optical system has excellent capabilit
To simulate the required set of soft X-ray projections, we used the algorithm described in Otón et al.4. The algorithm assumes totally incoherent illumination using the 3D point spread function (PSF) that characterizes the optical system of the microscope. In the case of Mistral microscope, ...