In microscopic imaging, transport of intensity equation (TIE) is an effective phase retrieval method. In order to effectively calculate the lost phase from the intensity information, a phase retrieval method based on the combination of traditional microscope monocular movement and TIE is proposed. In...
The diameter of the field in an optical microscope is expressed by the field-of-view number, or simply the field number, which is the diameter of the view field in millimeters measured at the intermediate image plane.
As previously discussed, the field ion microscope is basically a projection microscope, the magnification of which is, under standard operating conditions, of several millions, and a resolution of a fraction of a nanometer. With such performances, true atomic resolution is potentially achievable. Indee...
Digital microscope for 2D and 3D imaging and analysis If in the above equation, the total visual magnification is replaced by the useful range of magnification with white light (λ = 550 nm) illumination, i.e., 500 x NA < MTOT VIS > 1,000 x NA[3], it can be seen that, to a ...
Strictly speaking, this equation is valid only under the assumption ofmonochromatic radiationin which the transmittance multiplication follows the exponential rule. We may now apply this equation to twospectral bandsin the 15 μm CO2band, denoted by 1 and 2. It follows that ...
Complex field imaging, which captures both the amplitude and phase information of input optical fields or objects, can offer rich structural insights into samples, such as their absorption and refractive index distributions. However, conventional image s
An epi-fluorescent widefield microscope system excites all fluorophors in a given imaging plane regardless of depth. Resultant light emission is imaged through the objective at varying levels of focus. Objects within the objective’s depth-of-field appear crisp and in-focus; objects far out...
Extending the depth of field (DOF) is especially essential in thick and 3D sample imaging. However, it's difficult to achieve both large DOF and high resolution in a zoom microscope. Currently, the use of optical sectioning to expand DOF still has the pr
More particularly, at least some implementations relate to one or both of dark field microscopy and wide field fluorescence microscopy. BACKGROUND The optical microscope remains an irreplaceable tool for life science research even today, because light is often the best way to examine living cells ...
The contrast of the system was also computed using the Contrast equation and the contrast values were founded above 46.55%. Rabha et al. [88] designed a wide field smartphone microscope with applications in three types of imaging: brightfield, oblique illumination darkfield (OIDF), and total ...