The immunohistochemistry protocol for NG was described by our previous reports24,45: Horizontal sections of 7 μm thickness were collected for later klb protein staining (anti-klb, 1:100, Santa Cruz, USA). Whereas, for the whole section visualization of NTS46, rats were anesthetized with 3%...
Horseradish peroxidase (HRP)-conjugated IgG secondary antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Fluorescein isothiocyanate (FITC)-conjugated IgG secondary antibody was obtained from Boyun Biotechnology (Nanjing, China). And 4′,6-Diamidino-2-phenylindole (DAPI) solution ...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
Mouse anti-goat IgG-HRP Santa Cruz Biotechnology Cat#sc-2354; RRID: AB_628490 Goat anti-rabbit IgG, HRP-linked Cell Signaling Technology Cat#7074; RRID: AB_2099233 Horse anti-mouse IgG, HRP-linked Cell Signaling Technology Cat#7076; RRID: AB_330924 Goat anti-rat IgG, HRP-linked Cell Sign...
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Rabbit polyclonalAnti-PGC1α Santa Cruz Cat #sc-13067; RRID:AB_2166218 GAPDH monoclonal antibody Proteintech Cat #HRP-60004; RRID:AB_2107436 Rabbit monoclonal Anti-p-AMPKα Cell Signaling Technology Cat #2535S; RRID:AB_331250 Rabbit polyclonal Anti-AMPKα Cell Signaling Technology Cat #2532S; ...
For labeling, the sections were incubated with primary antibody overnight at 4 °C: anti-klb (Santa Cruz, USA) at a dilution of 1:200 in blocking solution. Then the sections were washed three times with PBST for 10 minutes each, and incubated in the appropriate secondary antibody (...
In parallel with an induction of FGF21, Thr172phosphorylation of AMPKα1 subunit, which is essential for AMPK activity, was robustly increased as determined by Western blot analysis using anti–phospho-AMPK antibody (8.1-fold, p < 0.01 at 1 h; 6.0-fold, p < 0.01 at 24 h)...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
FGF21 antibody goat FGF21 (Santa Cruz sc-16842), and secondary donkey anti goat IgG-HRP (Santa Cruz sc-2020). For detection the chemifluorescence detection method was used. The result was that in the wild trype strain FGF21 migrated as a double band. When PMT4 was deleted it had no ...