Technology) according to the manufacturer's protocol with an antibody against AFF1 (Bethyl), AFF4 (Abcam) or the control normal rabbit IgG (Santa Cruz Biotechnology). After dissociating the DNA-protein complexes, the pulled-down DNA and input DNA were subject to quanti- tative RT-PCR ...
Can Get Signal (Toyobo, Tokyo, Japan) was used to promote the reaction between primary antibody and antigen. Images were captured using a Konica SRX-201 (Konica, Tokyo, Japan). Densitometry measurements were carried out on selected scanned Western blot images, using the CS analyzer version 3 ...
SUMO1 Antibody Mouse Monoclonal (30 assays) (Agarose bead conjugate) SKU ASM11-beads Datasheet MSDS Amount 3 x 400 ul (Cat. # ASM11-Beads) Submit Order RequestContact Distributor As part of the Signal-Seeker™ product line, SUMOylation 1 affinity beads have been optimized in order to de...
a–cKeratinocytes expressing HA-CDK9 were treated with DMSO control, KL, or TPA. Immunoprecipitation using the HA antibody was performed from the lysate of these keratinocytes, followed by western blot probing for HA and HEXIM1.d–gViolin and ECDF plots showing HA-CDK9 traveling ratio (TR) ...