Nuclear marker DAPI is in blue and PLA signal is in red. Scale bar, 20 μm. b. Statistics of PLA dots/DAPI for PLA assay (a). Mean ± s.d., n = 12 ROIs containing 226 cells for DMSO, 216 cells for CGI1746, 184 cells for erastin, 222 cells for CGI1746+erastin. ...
cells were then rinsed three times with PBS and stained in a PBS solution contains cellular membrane marker DiI (20 μM) or Zn2+-specific fluorescent dye TSQ (30 μM) for 30 min. The cells were examined under a super-resolution confocal...
Conversely, 2-DG mitigated the ferroptosis indicators that were diminished by SPC25 downregulation, including the reduction of ferroptosis marker protein expression. By upregulating glycolysis in PRAD cells, SPC25 suppresses the occurrence of ferroptosis.Mingqiang Su...
AGO enrichment analysis of differentially expressed genes;BKEGG enrichment analysis of differentially expressed genes;CExpression of HSPA6, HSPA1A, HSPA1B, and HSPB1 in correlation with T cell exhaustion marker genes in pan-cancer tumor tissues, with values indicating the ratio of gene expression in...
6I). MDA is a well-established marker of lipid peroxidation, a hallmark of ferroptosis. Taken together, these findings imply that Klotho deficiency can activate intracellular ferroptosis, thereby contributing to cellular senescence. To clarify the upstream and downstream relationship between ABI3BP and...
CBS has been reported to be involved in ferroptosis as a marker of transsulfuration pathway activity and protects fibrosarcoma and liver cancer cells from ferroptosis[29],[30]. We wondered whether ferroptosis is involved inCBSLR-CBS signaling axis-mediated chemosensitivity. We found that downregulation...
(Figure 1A–D). After laser injury, we then examined the protein-expression level of 4-HNE, an oxidative stress marker. As shown inFigure 1E, the 4-HNE protein levels in the RPE/choroid complex of the CNV mice significantly increased to 1.9 times (p< 0.01) compared with the untreated ...
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1D). Finally, the expression of the ferroptosis marker acyl-CoA synthetase long-chain family member 4 (ACSL4) was elevated in the Hulth group and decreased after pioglitazone treatment (Fig. 1E). These findings suggest that PPARγ activation has a chondroprotective effect in osteoarthritis and ...
Therefore, we detected the expression of cell senescence marker β-galactosidase and P21 in different treatment groups (Additional file 1: Fig. S4A and B), and found that B-exos and MB-exos down-regulated the expression of damage-induced β-galactosidase and P21, and MB-exos had stronger ...