The PCR product was cloned into the similarly digested pTP1 expression vector. In pTP1 the thrombin cleavage site of the pET28b vector (Novagen) is replaced by the TEV protease cleavage of the pProEx HTa vector (Life Technologies, Darmstadt, Germany) and also the multiple cloning site was ...
The MHF1 DNA fragment was cloned into a modified pET-28a ( + ) vector without the thrombin cleavage site using the NdeI/XhoI restriction site. The DNA fragments of MHF2 and FANCM-F were inserted into another modified pET-28a ( + ) with a MBP–TEV cleavage site tag in the N...
The amplified TbPEX4FL gene was first inserted in the cloning vector pJET1.2 (Thermo Fisher Scientific) and from there transferred to the bacterial expression vector pET28a (Novagen) between the restriction sites NdeI and BamHI. This vector directs the expression of an N-terminally (His)6-...
1. The vector pET20b-CelA codes for a protein with theoretical molecular weight (MW) of 37.8 kDa and isoelectric point (pI) 5.49, while pET20b-CelA-linker-CBM11 codes for a protein with theoretical MW of 62.7 kDa and pI 5.42. Both the recombinant proteins were produced in soluble...
(Twist Bioscience HQ, San Francisco, CA, USA). The synthesized genes were cloned into the pET-28a(+) vector controlled by the T7 promoter. The constructed vectors were transformed and expressed inE. coliBL21 (DE3). The RrA gene was isolated from the bacteriumRhodospirillum rubrum(collection ...
To reduce feature vector dimension, the classification and regression tree (CART) was adopted to analyze the importance of each feature vector. Among the fifteen feature vectors, ten important feature vectors were extracted for further analysis. Thereafter, to further reduce feature vector dimension, ...
The enzymatic degradation of seaweed polysaccharides is gaining interest for its potential in the production of functional oligosaccharides and fermentable sugars. Herein, a novel alginate lyase, AlyRm3, was cloned from a marine strain, Rhodothermus mari
The initial 2D X-ray images were converted into 1D diffractograms as a function of the inverse scattering vector, s = 1/d = 2 sin θ/λ, by means of pyFAI python code (ESRF), modified by ALBA beamline staff. Film samples of around 5 × 5 × 0.1 mm were used in the synchrotron ...
Their method uses local detectors for each feature and combines their output with a set of global models for the part locations computed from a labeled set of examples. To model the appearance of each feature, a sliding window detector based on support vector machine (SVM) classifier with gray...