Htseq Count To Fpkm 由公式可知,知道了featurecount count 矩阵,同时有基因长度信息,可以计算RPKM.FPKM= read counts / (mapped reads (Millions) * exon length(KB))目前最关键是如何计算 基因长度 ,以及如何衡量基因长度。我们就能理解目前主流定义 基因长度 的几种方式。下面列举的是 非冗余外...
fpkmToTpm <- function(fpkm) { exp(log(fpkm) - log(sum(fpkm)) + log(1e6)) } countToEffCounts <- function(counts, len, effLen) { counts * (len / effLen) } 2. python 可能代码 importnumpyasnpimportpandasaspd A=pd.DataFrame({"gene_length":[2,4,1,10],"sample1":[10,20,5,0...
python featurecount_2_fpkm_tpm.py -h usage: --- This is a little script to calculate the fpkm and tpm. The inputfilemust be featurecounts resultfile---ifyou have any question please contact me,email:albert_xin@qq.com[-h][-i][-o FILE_OUT]optional arguments: -h, --help show thish...
python featurecount_2_fpkm_tpm.py -h usage: --- This is a little script to calculate the fpkm and tpm. The inputfilemust be featurecounts resultfile---ifyou have any question please contact me,email:albert_xin@qq.com[-h][-i][-o FILE_OUT]optional arguments: -h, --help show thish...