Here, we present a high-throughput sequencing method that allows the mapping of in vivo DROSHA cleavage sites at single nucleotide resolution, termed f ormaldehyde c ross l inking, i mmuno p recipitation, and seq uencing (fCLIP-seq). The fCLIP-seq protocol has been improved significantly ...
Thus, relying solely on binding sites determined by CLIP-seq from different cells or conditions can lead to a high false negative rate. This shortcoming can, however, be circumvented by applying models that predict additional putative binding sites. 参考文献:http...
CLIP-Seq用于检测RNA分子上的结合蛋白质,涉及结合反应的共价交联,接着通过RNA分子的酶切和纯化来进行CLIP-Seq。而在m6A-Clip-Seq中,则需要使用甲基化内切酶将RNA分子上的m6A修饰物识别出来,在RNA上形成裸核Ribonucleoside和基本的RNA结构,然后通过解压并钝化终止的加仑核苷,处理,并通过转录组方法进行测序。 通过对比m6A...
作者: Jérme Saulière,HL Hir 展开 摘要: UV cross-linking and immunoprecipitation coupled to high-throughput sequencing (CLIP-seq) is used to characterize RNA targets of RNA binding proteins (RBP) in a large scale manner. This powerful... 展开 关键词: Ribonucleoproteins Exon Junction Compl...
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CLIP-Seq protocols such as PAR-CLIP, HITS-CLIP or iCLIP allow a genome-wide analysis of protein-RNA interactions. For the processing of the resulting short read data, various tools are utilized. Some of these tools were specifically developed for CLIP-Seq data, whereas others were designed fo...
[Methods in Molecular Biology] Circular RNAs Volume 1724 || Discovering circRNA-microRNA Interactions from CLIP-Seq Datadoi:10.1007/978-1-4939-7562-4_16Dieterich, ChristophPapantonis, Argyris