1.1. Fatty acid chain elongation synthesis, which is remarkably active in eel-liver microsomes, requires [2-14C] malonyl-CoA as the two-carbon donor. NADPH is much more effective than NADH as reducing agent.2.2. Maximum elongation activity was obtained at an ATP concentration of 0.5 mM, ...
Microsomal fractions from rat small intestine contain a fatty-acid chain-elongation activity. Cofactor requirements are similar to those of the liver microsomal system, but substrate specificity is different. The polyunsaturated arachidonic and timnodonic acids were elongated at very low rates. These res...
Fatty acid elongation in developing seeds of Arabidopsis thaliana Six mutants of Arabidopsis thaliana (L.) Heynh., with reduced levels of very long chain fatty acids (VLCFAs) in their seed lipids, were isolated by direct ... L Kunst,D Taylor,EW Underhill - 《Plant Physiology & Biochemistry...
Fatty acid elongation genes and uses thereof 优质文献 相似文献 参考文献 引证文献Elongase genes and uses thereof The subject invention relates to the identification of several genes involved in the elongation of polyunsaturated fatty acids (i.e., "elongases") and to uses thereof. At least two of...
The biosynthesis of ‘unusual’ fatty acids with structures that deviate from the common C16 and C18 fatty acids has evolved numerous times in the plant kingdom. Characterization of unusual fatty acid biosynthesis has enabled increased understanding of e
According to a GC–MS analysis, the main components of F1 are fatty-acid derivatives: palmitic acid methyl ester, palmitic acid ethyl ester, octadecenoic acid methyl ester, linoleic acid ethyl ester, and oleic acid ethyl ester. Keywords: Agave angustifolia; edible plant; fatty acids; rheumatoid ...
Low erucic acid rapeseed (LEAR) is characterised by a near absence of very long chain fatty acids in the seed oil which has been correlated with a lack of acyl-CoA elongation activity. Here we show that the absence of acyl-CoA and ATP-dependent elongation activities in microsomes isolated fr...
We observed that the presence of microbes increases desaturation of palmitate (FA 16:0n-7) by SCD1 and elongation of γ-linoleic acid (FA 18:3n-6) to dihomo-γ-linolenic acid (FA 20:3n-6) by ELOVL5. Interestingly, SCD1 activity has been linked to various diseases including diabetes, ...
ECERIFERUM2-LIKE Proteins Have Unique Biochemical and Physiological Functions in Very-Long-Chain Fatty Acid Elongation1[OPEN] ECERIFERUM2-LIKE Proteins Have Unique Biochemical and Physiological Functions in Very-Long-Chain Fatty Acid Elongation1[OPEN]Figure 1. Expression of CER2-... TM Haslam,R ...
To determine the selectivity of the inhibitors identified during screening efforts we developed gel-elongation assay using crude bacterial lysate directly to determine the target specificities of fatty acid synthesis inhibitors (refs. 7,8).doi:10.1038/nprot.2006.134Kodali...