Acetyl-CoA synthetase probably has a bimodal distribution in mammalian cells, being found in both mitochondria1 and supernatant fractions of rat liver, kidney, heart, and adipose tissue. The most highly purified enzyme has been obtained from ox heart mitochondria by two different methods. Unstable,...
The greatest activity was observed with the acyl-CoA substrate. Significant activity (˜10% of that with acyl-CoA) was observed with acyl-ACP, but no activity was detectable with the N-acetyl-S-palmitoyl cysteamine substrate.D. Effectors of Activity...
Long-chain fatty acids (LCFAs) play important roles in cellular energy metabolism, acting as both an important energy source and signalling molecules1. LCFA-CoA esters promote their own oxidation by acting as allosteric inhibitors of acetyl-CoA carboxyla
Once fatty acyl-CoA is in the mitochondrial matrix, it is decomposed through β-oxidation to form acetyl-CoA [9]. During this process, the dehydrogenation performed by acyl-CoA dehydrogenases can also be a rate-limiting step [90]. The rapidly increased influx of FFAs in hepatic cells during...
This substrate flexibility extends further such that these proteins also efficiently utilize N-acetyl cysteamine, the shorter acceptor terminal portion of CoASH, to produce acyl-SNACs. We show that the in situ generated acyl-CoAs and acyl-SNACs could be channeled to types I and -III PKS systems...
In plants, light-dependent activation of de novo fatty acid synthesis (FAS) is partially mediated by acetyl-CoA carboxylase (ACCase), the first committed step for this pathway. However, it is not fully understood how plants control light-dependent FAS regulation to meet the cellular demand for ...
acyl-CoA chain [1]. Therefore, we tested overexpression of the nativeACL1to increase cytosolic acetyl-CoA production, overexpression of the nativeACC1to increase malonyl-CoA production from acetyl-CoA, and overexpression of three NADPH-producing genes (R. toruloidesglucose-6-phosphate dehydrogenase ...
体内、外模型证实枸杞多糖通过激活沉默信息调节因子1(silent information regulator of tranion 1,SIRT1)/肝激酶B1(liver kinase B1,LKB1)/AMPK通路增加乙酰辅酶A羧化酶(acetyl CoA carboxylase,ACC)磷酸化和脂肪三酰甘油脂肪酶(adipose triglyceride lipase,ATGL)蛋白表达,并降低脂肪酸合酶(fatty acid synthase,FAS)...
Fatty acids are synthesized in cytoplasm with the participation of ATP, NADPH, biotin, HCO 3 , and Mn++. Acetyl-CoA is the two-carbon building block molecule that is added to malonyl CoA (formed by carboxylation of acetyl-CoA). The enzyme catalyzing this reaction is the regulatory enzyme of...
Hormonal regulation of fatty acid synthetase, acetyl-CoA carboxylase and fatty acid synthesis in mammalian adipose tissue and liver. The major objectives of this study were to define the roles of adrenal glucocorticoids and glucagon in the long-term regulation of fatty acid synthetase an... JJ Vol...