Sharkova has purified acetyl-CoA synthetase from rabbit heart by the earlier method to a specific activity of 12 渭moles/min/mg at 37掳.doi:10.1016/S1874-6047(08)60146-8John C. LondesboroughLeslie T. Webster JrELSEVIEREnzymesLondesborough, J. C., and L. T. Webster, Jr. 1974. Fatty ...
In addition, wax synthase activity towards various acyl-thioester substrates was similarly tested using palmitoyl-CoA, palmitoyl-ACP and N-acetyl-S-palmitoyl cysteamine as acyl substrates. The greatest activity was observed with the acyl-CoA substrate. Significant activity (˜10% of that with acyl...
LCFA-CoA esters promote their own oxidation by acting as allosteric inhibitors of acetyl-CoA carboxylase, which reduces the production of malonyl-CoA and relieves inhibition of carnitine palmitoyl-transferase 1, thereby promoting LCFA-CoA transport into the mitochondria for β-oxidation2,3,4,5,6....
This substrate flexibility extends further such that these proteins also efficiently utilize N-acetyl cysteamine, the shorter acceptor terminal portion of CoASH, to produce acyl-SNACs. We show that the in situ generated acyl-CoAs and acyl-SNACs could be channeled to types I and -III PKS systems...
Free fatty acids (FFAs) in the cytoplasm are converted into fatty acyl-CoA, which can be transferred to the mitochondria and broken down through β-oxidation to generate acetyl-CoA. Increased FFA influx results in insufficient hepatic β-oxidation, and as a consequence, lipotoxic intermediates accu...
Fatty acyl-CoA reductase fromMarinobacter aqueoleiwas co-expressed with SpCas9 inR. toruloidesIFO0880 and a panel of gene overexpressions and Cas9-mediated gene deletions were explored to increase the fatty alcohol production. Two overexpression targets (ACL1andACC1, improving cytosolic acetyl-CoA and...
ACETYL-CoA carboxylaseFATTY acidsPLANT life cyclesMOLECULAR dockingIn plants, light-dependent activation of de novo fatty acid synthesis (FAS) is partially mediated by acetyl-CoA carboxylase (ACCase), the first committed step for this pathway. However, it is not fully understood how plants control ...
Fatty acids are synthesized in cytoplasm with the participation of ATP, NADPH, biotin, HCO 3 , and Mn++. Acetyl-CoA is the two-carbon building block molecule that is added to malonyl CoA (formed by carboxylation of acetyl-CoA). The enzyme catalyzing this reaction is the regulatory enzyme of...
Hormonal regulation of fatty acid synthetase, acetyl-CoA carboxylase and fatty acid synthesis in mammalian adipose tissue and liver. The major objectives of this study were to define the roles of adrenal glucocorticoids and glucagon in the long-term regulation of fatty acid synthetase an... JJ Vol...
Fatty acyl-CoAs of various chain lengths dose-dependently inhibited acetoacetyl-CoA synthetase from rat liver, but much less effectively a similar enzyme from a bacterium, Zoogloea ramigera I-16-M. Palmitoyl-CoA, the most potent inhibitor of the rat liver enzyme, with an apparent K i value ...