Fate mapping is also used to study cell migration and differentiation during development, as well as regeneration and repair during adulthood. This video will provide an overview of fate mapping, explain a protocol used to generate a fate map in zebrafish, and show some ways in which this ...
“细胞系图谱/跟踪”。自己编的啊，因为fate在遗传学中有“发育过程中，细胞将分化成的细胞类型”的意思，map有动词和名词意义，你看着选一种？我觉得它用动名词形式应该是偏向动词意义多一些，所以我偏向后一种 fate mapping命运映射.很高兴为你解答！如有不懂，请追问。 谢谢！谱系示踪。flox cre e...
中科大fate mapping原理: Cre-loxp系统: Cre酶可以切掉两个loxp位点之间的靶基因,通过控制cre基因的启动子可以实现细胞特异性敲除靶基因。例如RIP是胰岛β细胞特有的,RIP cre就是在β细胞里特异性敲除靶基因。 这个鼠是通过三种鼠杂交而得的。 第一种鼠 :ER是泰莫西芬受体,加入泰莫西芬才会表达cre酶,并且是β细胞...
The interpretation of fate-mapping studies depends upon multiple assumptions: (i) that expression of cre recombinase has no effect on monocyte-macrophage homeostasis, (ii) that tamoxifen is a neutral agonist, (iii) that life in an SPF animal facility reflects the normal life course of ...
Lineage tracing, also called fate mapping, is a technique that traces the appearance of heritable labels in different populations to study precursor to progeny relationships [41] and has been frequently used within the hematopoietic system to determine the developmental pathways of various hematopoietic ...
(https://cellrank.org) for single-cell fate mapping in diverse scenarios, including regeneration, reprogramming and disease, for which direction is unknown. Our approach combines the robustness of trajectory inference with directional information from RNA velocity, taking into account the gradual and ...
文章的题目是:Next-Generation Lineage Tracing and Fate Mapping to Interrogate Development,首先这个Next-Generation就会有一种面向未来的感觉。 Lineage tracing and fate mapping, overlapping yet distinct disciplines to follow cells and their progeny, have evolved rapidly over the last century. Lineage tracing...
loxp位点,故当表达cre酶后,STOP位点被切除,细胞表达YFP。 第三种鼠:DTR鼠,DTR是白喉毒素受体,只在灵长类里存在,注 射DT(白喉毒素)后会死亡,但是小鼠这种啮齿类是不表达DTR的,基因操 作后使小鼠表达RIP的细胞即β细胞表达DTR,注射DT后,β细...
Antibody dynamics resulting from sequential immunization are complex, limiting the study of concepts such as ‘original antigenic sin’. Here, molecular fate-mapping defines an ‘addiction’ of boosted antibodies to primary clones, and OAS-like suppressi
针对缺氧肿瘤细胞具有活性氧(reactive oxygen species , ROS)耐药表型的特性,未来可能有助于预防或限制肿瘤细胞的转移。 原文检索: Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis. DOI:https://doi.org/10.1038/s41467-019-12412-1...