Fate mapping is also used to study cell migration and differentiation during development, as well as regeneration and repair during adulthood. This video will provide an overview of fate mapping, explain a protocol used to generate a fate map in zebrafish, and show some ways in which this ...
“细胞系图谱/跟踪”。自己编的啊，因为fate在遗传学中有“发育过程中，细胞将分化成的细胞类型”的意思，map有动词和名词意义，你看着选一种？我觉得它用动名词形式应该是偏向动词意义多一些，所以我偏向后一种 fate mapping命运映射.很高兴为你解答！如有不懂，请追问。 谢谢！谱系示踪。flox cre e...
中科大fate mapping原理: Cre-loxp系统: Cre酶可以切掉两个loxp位点之间的靶基因,通过控制cre基因的启动子可以实现细胞特异性敲除靶基因。例如RIP是胰岛β细胞特有的,RIP cre就是在β细胞里特异性敲除靶基因。 这个鼠是通过三种鼠杂交而得的。 第一种鼠 :ER是泰莫西芬受体,加入泰莫西芬才会表达cre酶,并且是β细胞...
Lineage tracing, also called fate mapping, is a technique that traces the appearance of heritable labels in different populations to study precursor to progeny relationships [41] and has been frequently used within the hematopoietic system to determine the developmental pathways of various hematopoietic ...
Antibody dynamics resulting from sequential immunization are complex, limiting the study of concepts such as ‘original antigenic sin’. Here, molecular fate-mapping defines an ‘addiction’ of boosted antibodies to primary clones, and OAS-like suppressi
图2. 用于生成hypoxia fate-mapping system的两个慢病毒载体 然后,研究人员创建了一种三联转基因小鼠(其中Cre转基因小鼠由赛业生物构建),在肿瘤进展期间将自发的乳腺癌发展为fate-map hypoxic cells,发现体外研究并不能再现体内瘤内缺氧的反应。在体内经历肿瘤内缺氧的细胞产生的基因表达特征与通过体外缺氧暴露建立的细...
Here we report fate mapping of hypoxic cells and their progenies by generating a transgenic mouse expressing a chimaeric protein in which the oxygen-dependent degradation (ODD) domain of Hif-1卤 is fused to the tamoxifen-inducible CreERT2 recombinase. In mice bearing the creERT2-ODD transgene ...
原理:Cre-loxp系统:Cre酶可以切掉两个loxp位点之间的靶基因,通过控制cre基因的启动子可以实现细胞特异性敲除靶基因。例如RIP是胰..
网络释义 1. 细胞发育制图技术 . 随后在体内肾脏、肝脏、肺、心脏组织中, 人们利用细胞发育制图技术(fate-mapping)技术, 证实了这一点 www.wjgnet.com|基于 1 个网页
Figure 1The Distinction between Fate Mapping and Lineage Tracing 2.Foundations of Fate Mapping and Lineage Tracing: Direct Observation and Cell Labeling 接着,作者总结了Lineage Tracing Techniques的发展历史。最开始的测量方法是:Observation Biology Era,中的染料注射来标记不同类型的细胞, 这主要是在1890s的...