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The resulting supernatant was loaded onto a Hitrap Excel column (5 mL, GE Healthcare) equilibrated with buffer, 50 mM Tris-HCl pH 8, 300 mM NaCl, 10% glycerol, and 5 mM imidazole. Bound proteins were eluted with a gradient of imidazole (0–500 mM) in buffer: 50 mM Tris-HCl pH 8...