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The enhanced chemiluminescence (ECL) reagent was obtained from GE Healthcare (Little Chalfont, UK). Recombinant human EGF, FGF, PDGF, and VEGF were obtained from Biovision (Milpitas, CA). IL-2 was provided by the National Institutes of Health AIDS Reagent Program (Germantown, MD). Cell ...
The trypsin-activated toxins were loaded into a HiTrap Q HP column connected to the fast protein liquid chromatography system (ÄKTA, GE Healthcare Life Sciences). The column was washed with Buffer A (50 mM NaCl, 0.05 mM CO3/HCO3, pH 8.5) followed by elution with a gradient from ...