A. The reaction driven by the FAD-dependent phenylalanine oxidase IL-4I1, leading to the generation of H2O2 (yellow highlight, right), is depicted. B. IL-4I1 activity (RFU, mean ± SD, n = 4) in the presence of either rSmNPP5 (red symbols) or control protein (BSA, ...
Direct electron transfer (DET) Glucose oxidase 1. Introduction The history of the development of glucose sensors goes hand-in-hand with the history of discovery and engineering of glucose-sensing enzymes [1]. The first reported electrochemical glucose sensor was developed by the combination of gluco...
The contents of cytochrome b, suggested as the terminal component of the oxidase electron transport chain, and FAD, the hypothetical proximal component of the chain, were determined in patients and in carriers. Cytochrome b showed the typical behaviour of x-linked CGD: total absence in patients,...
Lysine-specific demethylase-1 (LSD1, also known as KDM1A) is a member of the flavin-containing amine oxidase family that, in general, represses transcription by removing the methyl group from mono-methylated and di-methylated lysine 4 of histone H3 (H3K4)6. LSD1 is also involved in the d...
Erv2p is an FAD-dependent sulfhydryl oxidase that can promote disulfide bond formation during protein biosynthesis in the yeast endoplasmic reticulum. The structure of Erv2p, determined by X-ray crystallography to 1.5 Å resolution, reveals a helix-rich dimer with no global resemblance to other kn...
CI–CIV, complexes I–IV; COX, cytochrome c oxidase; CS, citrate synthase; EM, electron microscopy; F, female; FADS, FAD synthase domain; fs, frameshift; M, male; MPTb, molybdopterin binding domain; NR, not reported; NT, not tested; PDH, pyruvate dehydrogenase; and SDH, succinate dehydr...
As gut metabolite alteration has been linked to a defect in mucosal integrity and immune balance19,21, we then asked whether the gut epithelium might be exposed to a proinflammatory environment due to metabolic disorders. Hematoxylin-eosin (HE) staining of ileum tissue sections from both WT and ...
The tree peony (Paeonia ostii), a newly recognized woody oil plant endemic to China, is noteworthy for its high content of unsaturated fatty acids (UFA), particularly alpha-linolenic acid (ALA). Fatty acid desaturases (FADs) are integral to plant develop
glucose dehydrogenase;glucose oxidase;GMC oxidoreductase;CAZy AA3_2;substrate specificity 1. Introduction The Carbohydrate-Active enZymes database classifies a number of enzymes involved in the transformation of carbohydrates based on their functional amino acid sequences, reaction mechanisms, and structural...
The monomer of HpaB consists of three domains and a C-terminal tail [94]. The N-terminal and BCio-ltoegrym20i1n8a, l7,dxomain contain mainly antiparallel α-helices, while the middle domain contains β-s8hoefe3t4s forming a barrel-like structure (Figure 4). A cave at the interspace ...