In the catalytic subunit of BcGDH, various amino acid sequences namely, the FAD binding motif (Gly-x-Gly-x-x-Gly), the amino acids around the substrate binding pocket, the and expected active site amino acids (His), are concerved, and these are the signature features of the glucose ...
Each of these proteins has a conserved dinucleotide-binding motif at the N-terminus. To determine if GidA binds dinucleotide, the M. xanthus gene was expressed with a His6 tag in E. coli cells. Purified rGidA is a yellow protein that absorbs maximally at 374 and 450 nm, consistent with ...
GXXG motif is also the first residue of a newly discovered motif, GXXXG/A, located on the a-helix. Together these two motifs stabilize FAD or NAD(P)-binding Rossmann folds and binding of the nucleotide cofactor to the domain.
Those motifs include an ADE (adenine binding) motif, two arginine containing motifs (Arg-1 and Arg-2), a γ-phosphate motif and a pyrophosphate loop (PP-loop) [39]. A flavin binding motif is boxed in red. Specific con- served residues known to be involved in substrate/prod- uct ...
pcDNA3-GAL4-hLSD1mut, which expressed the FAD-binding motif-mutated LSD1, was prepared by site-directed mutagenesis. Two glycine-to-alanine mutations were introduced using the primer pair listed in Supplementary Table S4. GAL4-binding motifs-containing reporter vector (pGL3-GALx5-SNRPN) was ...
MBS TTTTTACGGTTA MYB binding site/drought-inducibility 66 STRE AGGGG Stress-Responsive Element 224 TCA-element CCATCTTTTT responsive to salicylic acid 88 TC-rich repeats GTTTTCTTAC defense and stress responsive 50 W box/WRE3 TTGACC expression of defense-related genes. 179 WUN-motif AAATTTCCT ...
This is probably why so many different proteins with different functions use this motif to bind an adenylate-containing ligand. The adenylate-binding motif reported is present in ancient proteins common to all living organisms, suggesting that adenine-containing ligands and the common motif for ...
(#). Five conserved structural motifs of SmFADS found in the PAPS catalytic domain are bounded by black boxes and named. A flavin binding motif is boxed in red. Residues involved in substrate/product interaction in the PAPS domain are indicated by ($). Key conserved residues in the enzyme...
ahfad2、ahfad3和ahfad7/8序列相对保守,motif 1和 motif 2中分别含有花生histidine box 3两端特有保守侧翼结构序列fxxxphyhxxea和ewxxlrgxlxt,motif 4和motif 5分别包含了histidine box 1(xhxcghx)和histidine box 2(wxxshrxhh)两个组氨酸富集区。fad4/5和fad6与其他膜结合fad亚家族差异明显,其中fad6家族 27...
The structure of Erv2p, determined by X-ray crystallography to 1.5 Å resolution, reveals a helix-rich dimer with no global resemblance to other known FAD-binding proteins or thiol oxidoreductases. Two pairs of cysteine residues are required for Erv2p activity. The first (Cys-Gly-Glu-Cys) ...