For each pooled extraction, the V4 region of the 16 S ribosomal RNA (16 S rRNA) gene was amplified in triplets using the universal primers 515 F and 806 R adapted with linker regions and barcoded se
MicroRNA (miRNA) transfection is known to degrade target mRNAs and to decrease mRNA expression. In contrast to the notion that most of the gene expression alterations caused by miRNA transfection involve downregulation, they often involve both up- and downregulation; this phenomenon is thought to be...