The subject of the present invention is the tomato nitrate reductase structural gene, as well as vectors for cloning and expression of the said gene and a process for enhancing the assimilation of nitrates in plants, characterised in that a DNA sequence or a vector according to the invention is...
cloning sites (mcs) of two different expression vectors, but can also be cloned into a dual-expression vector with two mcs, so both the heavy and light chains are expressed by the same construct. For the two-vector method, select twovectors with the same antibiotic resistance before choosing...
This vector was modified to include the described signal peptides, 5′UTRs, other restriction sites and the indicated inserts by common cloning techniques. The vector backbone was not modified and contains the OpiE2 promoter, the IE1 terminator and surrounding enhancing regions of the polH region...
(Eppendorf). Twenty-four hours after seeding, cells were transfected with 50 ng per well of either reporter plasmid pMIR-RNL-TK, with or without insert, and 200 ng per well of miRNA expression plasmid pSG5-miR-29a or the empty expression vector pSG5. Forty-eight hours post-...
In the present study we demonstrate the first cloning, localization, and functional expression of two Na+ channel β1 orthologs in zebrafish, scn1ba_tv1 and scn1ba_tv2, which arise from alternative splicing of scn1ba. We also show, for the first time, the functional expression of a ze...
3.1. The choice of expression host and vector 3.1.1. Expression of bacterial LPMOs In general, bacterial (prokaryotic) LPMOs are commonly expressed using the Gram-negative bacterium Escherichia coli (Table 1 and Supplementary Table S1) via periplasmic expression (Fig. 2A). Among the different E...
Cloning, Heterologous Expression of ApDXS in E. coli, and Identification of Gene Product by IPTG Induction According to restriction site analysis, the ORF of the ApDXS gene of A. paniculata was inserted into expression vector pET-32a ( +) between Not I and Kpn I restriction sites. The ...
Sexual dimorphic expression of putative antennal carrier protein genes in the malaria vector Anopheles gambiae. Insect Mol Biol. 2003;12(6):581–94. Article CAS PubMed Google Scholar Pelosi P, Iovinella I, Felicioli A, Dani FR. Soluble proteins of chemical communication: an overview across ...
With the pGB vector series, we could create various combinations of these genes by first cloning each gene individually into a specific pGB vector, and then combining all desired expression plasmids into a single construct via a single step Golden Gate reaction. In these initial experiments, the ...
Conceptual design of the cloning toolkit The construction of a vector for the expression of fusion proteins requires the subcloning of the cDNA of interest into a vector’s backbone, at a site next to one or more pre-existing in-frame modules that provide the functions to be monitored. Hence...