1. In the previous report, the effect of the varied day length and night temperature of two or four days duration upon the sex pattern of the cucumber plant was studied. It is observed that long day and high night temperature of even such short duration influenced the physiological state aro...
we established the utility of another widely used animal model, the Japanese quail (Coturnix japonica), for clarifying the molecular mechanisms underlying gonadal sex differentiation. In particular, we performed comprehensive gene expression profiling of embryonic gonads at three stages (HH27, HH31 and ...
In situ hybridization revealed that MIF mRNA expression was significantly up-regulated in the whole brain by intracranial JEV inoculation at 2 days post-inoculation (d.p.i.). Neurons as well as glial cells expressed MIF transcripts in which some of these cells were co-labeled by double ...
Haplodiploid parasitoid species are important insects that parasitize other organisms [34] and have been extensively applied to reduce the population sizes of pest species [35,36,37,38,39,40]. However, sex differences in gene expression have not been investigated in parasitoid species other than ...
In this study, bmp2b expression levels in fins of adult Japanese medaka (Oryzias latipes) after treatment with sex steroid hormones were examined using semi-quantitative RT-PCR. In males, bmp2b expression levels in the posterior part of anal fins were increased at6 days after 11-ketotestosterone...
5 days for myotube formation. Full differentiation was determined via visual confirmation where approximately 70% of the myotubes should have > 3 nuclei. DNA and RNA was extracted from the cells using the DNeasy blood and tissue kit (Qiagen, Hilding, Germany) and Trizol in combination ...
Using the to investigate stretch-induced hypertrophy, we demonstrated an increase in the expression of fast in the predominantly slow anterior latissimus dorsi muscle (). The fast content of the control quail is not sufficient to be quantified on native gels. After 33 days of stretch, the fast...
Rinx mRNA was also analyzed by reverse transcription-polymerase chain reaction in the lens. At P0 and P4 (postnatal days), Rinx-immunoreactive cells included retinal ganglion cells (RGC), cells of the innermost nuclear layer (INL) and of presumptive INL, differentiating lens fiber cells, and ...
Japanese encephalitis virus (JEV) is a plus strand RNA virus, which infects brain. MicroRNAs are regulatory non-coding RNAs which regulate the expression of various genes in cells. Viruses modulate the expression of various microRNAs to suppress anti-viral signaling and evade the immune response. ...
(not in exact scale) marked by negative (-) signs represent the days before the onset of skin disease. #3 indicates Tg mouse #3, #6 indicates Tg mouse #6. In (c), total serum levels of IgE in relation to the progression of skin disease (as indicated by severity scores) in seven ...