Transgenic mice can be produced in several ways鈥攎ost common method is micro-injection of DNA into the male pronucleus of a zygote. Ig transgenes are expressed as expected from the transcriptional promoters and enhancers included in the transgene. Increased levels of B-cells with a single ...
Sclerostin inhibits Wnt signalling and can also inhibit downstream effects of BMP such as alkaline phosphatase (ALP) activity and matrix mineralisation in vitro. The regulation of sclerostin or SOST, the gene encoding sclerostin, is not completely clear. Therefore, we investigated a possible feedback...
The regulation of Sost gene expression by HIF-1α was then investigated. We performed loss-of-function experiments to examine Sost expression by using siRNA approach against HIF-1α, and found that the inhibition of HIF-1α by siRNA in osteoblasts led to the decrease of Sost expression. ...
In this study, the transcriptome was used to illustrate the mechanisms of gene expression regulation in the somatic embryogenesis of tea plants. The number of DEGs for the (IS intermediate stage)_PS (preliminary stage), ES (embryoid stage)_IS and ES_PS stages were 109, 2848 and 1697, ...
Co-expression analysis also revealed that OsNF-Y genes might interact with each other, suggesting that NF-Y subunits formed complexes that take part in transcriptional regulation. These results provide useful information for further elucidating the function of the NF-Y family and their regulatory ...
It is partially owing to the base number of detected circRNAs and miRNAs in the microarray is smaller. However, we could still figure out that there exists a core circRNA-miRNA regulation network during the process of osteoclastogenesis. In our results, all of the differentially expressed circRNAs...
heteroclitus that live in different thermal regimes appear to compensate via differences in the transcriptional regulation of the Ldh-B locus. The transcriptional regulation of eukaryotic genes is the result of the interaction of protein transcription factors with their cognate binding elements in D...
Gain-of-function studies showed that the upregulation of miR-124 in OS cells was significantly correlated with the proliferation, invasion and tumor growth of OS cell lines. miR-124 significantly decreased tumor volume and weightin vivo. Bioinformatics analysis predicted that snail family zinc finger...
The resulting clone, SKA3SH8Z11NA1, was digested with Xho I and Not I to release the construct from vector sequences before microinjection. Deletion constructs were made with different fragments of the scleraxis gene and a lacZ gene under the regulation of the HSp68 promoter (Kothary et al...
Next to these established pathways, new avenues of research can be opened with unbiased gene expression profiling as a first step toward addressing potential metabolic implications that were previously unknown. Carnitine palmitoyltransferase I (CPT-I) is a key enzyme in the regulation of the ...