We generated 1510 expressed sequence tags (ESTs) from a mycelial cDNA library ofS. parasitica. A total of 1279 consensus sequences corresponding to 525944 base pairs were assembled. About half of the unigenes showed similarities to known protein sequences or motifs. TheS. parasiticasequences tende...
Expressed Sequence Tag Simple Sequence Repeats (EST-SSRs), also known as transcriptome-derived SSRs, are microsatellite markers extracted from expressed sequence tags (ESTs) of transcriptome RNA sequences located explicitly in the exons of cDNA sequences. Compared to microsatellite sequences located in...
An Expressed Sequence Tag (EST) database constructed using cDNA libraries from immune tissues [6, 7] has been recently enriched using new generation sequencing (NGS) technologies and currently contains 35,000 contigs and 65,000 singletons. This database was used to design the first turbot oligo...
Therefore, we expected the nam-S227 allele also to harbor a dTph1 insertion. We cloned nine distinct dTph1 flanking sequences that were unique for a family of nam-S227 plants (Souer et al. 1995), one of which (clone es87) fully linked to the nam locus in the 18 NAM+/nam− and ...
The gene expression profiles that we obtained by tag profiling yielded 2.4–3.6 million signature sequences (Tags) per data set for the NPF, CAF and fetal (embryonic, EMB) samples (available at GEO GSE25018). Each tag library was filtered to include only well-annotated tags (assigned class...
This protein exhibits a high degree of sequence similarity with CD33 and contains all of the key structural features of siglecs described above. As predicted, siglec-5 is able to mediate sialic acid–dependent binding to cells either when expressed on COS cells or as a recombinant protein ...
These sequences were used as queries to screen the wheat Expressed Sequence Tags (EST) database using the Basic Local Alignment Search Tool (BLAST) (Altschul et al. 1997). Best-matching wheat ESTs with an E-value lower than 1E-70 were assembled using the software Contig-Express in Vector...
SEQUENCE USAGE Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. STORAGE Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. STABILITY ...
In another embodiment, the invention provides a subtraction hybridization differential display method to identify, isolate, and clone expressed sequence tags (ESTs) of mRNA species encoding rare proteins, such as those involved in telomere elongation and the regulation of telomere length and telomerase ...
2. Portions of the 84P2A9 amino acid sequence show some homologies to ESTs in the dbEST database. The prostate and testis-related expression profile of 84P2A9 in normal adult tissues, combined with the over-expression observed in prostate tumor xenografts, shows that 84P2A9 is aberrantly over...