analysisofexpressedsequencetags JochenKleemann,HiroyukiTakahara,KurtStu¨berandRichardO’Connell Correspondence RichardO’Connell oconnel@mpiz-koeln.mpg.de Max-Planck-InstituteforPlantBreedingResearch,DepartmentofPlant–MicrobeInteractions, D-50829Ko¨ln,Germany ...
Turbot marker sequences were also used as queries for analysis against the stickleback cDNA database, as the most informative model genome in this study (see Results). We wrote a BioPerl BLAST parser to extract the desired hits including sequence similarity figures and genome location information ...
Expressed sequence tags(ESTs), the short segments produced by partial sequencing ofcDNA clones, are being generated rapidly in huge quantities; they now outnumber all other publicly available sequence and thus provide a considerable information resource. However, these data are not ideal: the sequence...
Harbin Insistitute of Technology, Harbin 1 5 0 0 0 1,P.R.China ABSTRACT The focus on genome research has shifted from structural genomics to functional annotation. A rapidly growing area of functional research is the generation of expressed sequence tags (EST) in which a large number of rand...
Expressed Sequence Tags (ESTs) represent the transcribed portion of an organism's genome and as such provide a snapshot of the expression of that genome at a particular time. Since non-coding DNA such as introns, intergenic spacers, repeat elements, etc. which comprise the bulk of the ...
A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences ...
The proteins have unusually high levels of glutamine and proline and contain large regions of repetitive sequences. While most gamma gliadins are monomeric proteins containing eight conserved cysteine residues, some contain an additional cysteine residue that enables them to be linked with other gluten...
Genomics resources for the zebra finch include >50,000 expressed sequence tags (ESTs), mostly generated as part of the Songbird Neurogenomics Initiative [33]. The aim of this paper is to demonstrate that microsatellite loci within zebra finch ESTs are a useful resource for population and ...
satsuma mandarin (Citrus unshiu cv. 'Miyagawa-wase')was characterized through the generation of 626 expressed sequence tags(ESTs)derived from a cDNA library prepared from albedo tissues collected 60days after anthesis.A Total of 451ESTs was found to have significant similarity to the known sequence...
tagsfromtheTheTagsFrom DNAResearch10,147–153(2003) ExpressedSequenceTagsfromtheClosterium peracerosum-strigosum-littoralecomplex,aUnicellular CharophyceanAlga,intheSexualReproductionProcess HiroyukiSekimoto, 1,2,∗ YoichiTanabe, 3,4 MariTakizawa, 1 NaoIto, 3 Ryo-heiFukumoto, 5 andMotomiIto 3 Instit...