Exosome Technology Exosomes are nano-sized, membrane-enclosed vesicles that released by nearly every cell type in the body. Exosomes contain various molecular constituents (genetic material and proteins) of their cell of origin, and act as powerful messengers between cells, ultimately providing a regul...
Exosome production is a highly regulated process occurring through either of two mechanisms, one dependent on and another independent of the endosomal sorting complex required for transport (ESCRT) [69,70]. ESCRT is well acknowledged as the primary regulator of early endosome development and late end...
Quantitative real time PCR (qRT-PCR) According to the manufacturer’s instruction total RNA was extracted from DOX and DDMSNP treated MDAMB-231 and 4T1 cells using TRIzol reagent (Invitrogen, NY, USA). For each sample, 2 μg of RNA was converted to cDNA using cDNA isolation kit (Bio-Ra...
The SYBR Green SuperReal PreMix Plus Kit (TIANGEN Biotech, Beijing, China) was used to perform a quantitative real-time PCR (qRT‒PCR) assay on a real-time fluorescence quantitative PCR system (CFX96, BioRad, Hercules, CA). GAPDH levels were used to normalize the disparities between ...
However, the function and regulatory mechanism of hsa_circ_0012634 in PDAC progression remain unclear. Quantitative real-time PCR was used to measure the expression of hsa_circ_0012634, microRNA (miR)-147b and homeodomain interacting protein kinase 2 (HIPK2). Cell function was assessed by cell...
(PFG) NMR Service Pure Shift NMR Service Quantitative NMR Service Real-time Protein NMR Service Rheo-NMR Service Saturation Transfer Difference (STD) NMR Service Stable-Isotope Aided NMR Service Solution-state NMR Service Other Microscopy Services+ Scanning Electron Microscope Service Transmission Electron...
real-time liquid biopsy biomarkers, which could provide a promising application for diagnosis and simultaneous monitoring. Advances in the exosomes field and a more profound understanding of the underlying function of exosomes would significantly lead to breakthroughs in clinical applications to benefit pati...
Real-time RT-PCR was conducted on QuantStudio™ 3 Real-Time PCR System (Applied Biosystems™). Primers listed in Additional File 3: Supplemental Table 3. Nanoparticle tracking analysis, NTA Exosomes were isolated using Total Exosome Isolation Reagent (Invitrogen, # 4478360) according to the ...
The uptake of Dil-labeled Exos and HExos by BV2 microglia was then observed by laser confocal microscopy and the fluorescence intensity of Dil was measured with ZEN lite software at different time points within the two groups. Vector constructs, lentivirus production, and cell transfections LV2-...
Quantitative real time RT-PCR Total RNA preparation and quantitative real-time PCR (qPCR) were performed as previously described49. The following primers were used: Murine Hprt Forward 5′-GAT TAG CGA TGA TGA ACC AGG TTA-3′; Hprt Reverse 5′-GAC ATC TCG AGC AAG TCT TTC AGT C-3′; ...