Unfortunately, a simple and reliable method to separate exosomes has yet to be developed. As can be expected, the lack of a standardized method for extraction and purification has contributed to suboptimal inter-laboratory correlation and difficulty in comparison studies. Traditional techniques such as...
the loading methods of therapeutic molecules, the efficiency of cell uptake of exosomes. Exosomes derived from different cell types have diverse functions. For example, human embryonic kidney (HEK293) cells have been widely used in the field of biopharmaceutical manufacturing owing to the advantages o...
Exosomes’ inherent characteristics offer them certain advantages in target cell absorption as compared to conventional nanomedicine delivery methods. However, additional changes are required to enhance exosomes’ capacity to target disease sites [24]. It is commonly recognized that the most advanced target...
As such, MVs are directed to the sides of channels, while exosomes stay in the middle of the channel.33 Exosomes Purification and Enrichment Technology Ultracentrifugation Differential ultracentrifugation or density gradient ultracentrifugation is the current gold standard to enrich and purify exosomes. ...
Exosomes, small extracellular vesicles secreted by cells, have emerged as focal mediators in intercellular communication and therapeutic interventions across diverse biomedical fields. Inflammatory disorders, including inflammatory bowel disease, acute liver injury, lung injury, neuroinflammation, and myocardial ...
technology is fast and requires only a small amount of sample and few reagents [41]. This technology is not only used to isolate exosomes, but it can also be used in the diagnosis, detection, and differentiation of cancers [42]. Tumour-related exosomes can be detected in the plasma using...
They are also extensively being used in cancer immunotherapy. On the other hand, exosomes are naturally occurring nanosized extracellular vesicles that serve an important role in cell–cell communication. Importantly, the exosomes also have proven their capability to carry an array of active ...
Cell Communication and Signaling (2023) 21:202 https://doi.org/10.1186/s12964-023-01227-9 Cell Communication and Signaling REVIEW Open Access Advances in the study of exosomes derived from mesenchymal stem cells and cardiac cells for the treatment of myocardial infarction Yuchang...
Exosomes, which are nanosized vesicles secreted by cells, are attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents in intercellular communicat...
Lung cancer (LC) is among the leading causes of death all over the world and it is often diagnosed at advanced or metastatic stages. Exosomes, derived from