Define intron-exon junction. intron-exon junction synonyms, intron-exon junction pronunciation, intron-exon junction translation, English dictionary definition of intron-exon junction. n. A segment of a gene situated between exons that is removed before
Abstract. Summary: ExPrimer is a web-based computer program to design primers mainly from a specified exon—exon junction (E-E-jn) of a gene of interest. Th
PartⅡCircInteractome数据库设计circRNA的siRNA的靶标序列及Divergent primers 1. circRNA验证反向引物设计: 进入CircInteractome主页(https://circinteractome.nia.nih.gov/),选择Divergent primers search;输入circRNA分子后,点击Divergent primers search,即可获得所查询circRNA分子的junction sequence,下面还附带了2个PCR引...
“primer set 1”. The RT-PCR bands show the size of the PCR product, and the band intensity does not represent relative expression levels. The junction in the circRNA produced by the circEIF3J_1 kb plasmid was different from that of circEIF3J, and this difference is shown in the gel ...
Exon-exon junctionPrimer designRT-qPCRAlternative splicingGene expressionWeb toolReverse transcription quantitative PCR (RT-qPCR) with intercalating dyes is one of the main techniques to assess gene expression levels used in basic and applied research as well as in diagnostics. However, primer design ...
N6-methyladenosine (m6A), the most abundant modification of mRNA, is essential for normal development and dysregulation promotes cancer. m6A is highly enriched in the 3’ untranslated region (UTR) of a large subset of mRNAs to influence mRNA stability an
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Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target...
The exon junction complex (EJC) is a dynamic multi-protein complex deposited onto nuclear spliced mRNAs upstream of exon-exon junctions. The four core proteins, eIF4A3, Magoh, Y14 and MLN51, are stably bound to mRNAs during their lifecycle, serving as a
Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and imm