Give some examples of frameshift mutation. (a) What chromosomal mutation is typically observed in CML patients and what is the name the new oncogene created by this mutation? (b) Which protein does this oncogene overproduce and how does this a...
New cases of FBS have been recently reported in the Middle and Far East in collaboration with specialized centers. Two novel mutations have been discovered in two unrelated Egyptian families. The first was two bases deletion, guanine and adenine, (c.253_254delGA) causing a frameshift mutation (...
This mutation causes the frameshift and induces premature translation termination. The resistance of this mutant suggests that the upper phage recognizes OmpC as a main receptor. The strains E. coli 1304PP2022 and 1306PP2022, which are generated by exposition to phage 303Ecol101PP, have a ...
(iii) tetra-primer amplification refractory mutation system PCR (ARMS-PCR) [11,12]. Of these, ARMS-PCR proved to be the most suitable. Thus, the method was optimized to further reduce handling steps and costs. The methods were summarized to provide an overview on handling and possible ...