The working plan involved 6 laboratories for the final statistic analyses, by the use of freeze-dried milk samples (adequately reconstituted on the basis of established methods) from Sardinia, Comisana, and Massese ewes. All the considered variability factors showed a highly significant effect (P...
The performance of commercially available shelf-stable milk powders that upon reconstitution can be used as calibration samples for mid-infrared (IR) analyzers was evaluated. Twelve reconstituted powders and 7 raw milk samples were sent to each of 7 laboratories. Each laboratory analyzed the reconstitu...
Laboratories performing sperm "counts", in general, vary in the details that they provide the physician requesting the "count". A general sperm count as part of a fertility evaluation should include the total density or count (20 million per ml or above), and the motile density (8 million ...
Evaluation of different methods for the detection and identification of Enterobacter sakazakii isolated from South African infant formula milks and the pro... Enterobacter sakazakii is an emerging pathogen associated with life-threatening neonatal infections resulting from the consumption of contaminated powde...
Ehrlich, R. (1955) Technique for microscopic count of microorganisms directly on membrane filters.J. Bacteriol.,70, 265–268. CASGoogle Scholar Entis, P. (1983) Enumeration of total coliforms, fecal coliforms andEscherichia coliin nonfat dry milk and canned custard by hydrophobic grid membrane fi...
A method performance evaluation and lessons learned after analyzing more than 5,000 human adipose tissue, serum, and breast milk samples for polychlorinate... we discuss the results of a performance evaluation of our method which includes small interlaboratory studies with other laboratories performing...
To resuspend the Freeze-Drying HBM, the initial solid: liquid ratio was used (11 % of solids). The aliquots were thawed in water and shaken at 45ºC until all milk was liquid (up to 5 min) (Zhang et al., 2022). Then, samples were vortexed to ensure homogenization. 2.3. ...
All other reagents; acetonitrile, methanol, water, and formic acid used were of HPLC grade. Study design Animal groups: Thirty-six male Wistar rats (185–220 g) were purchased from the animal house laboratories- Applied Science Private University-School of Pharmacy (Amman, Jordan). Rats...
The samples were blocked at room temperature for 1 h in PBS containing 0.05% Tween-20 (PBS-T) containing 2% skim milk and incubated with primary antibodies at 4 °C overnight. After washing with PBS-T, the membranes were incubated with HRP-conjugated secondary antibodies at room ...
In calculating energy requirements, all systems recognize that NE requirement (NEr) of beef cattle and sheep is the sum of their requirements for maintenance (NEm), production [milk production (NEl), liveweight gain (NEg), and fleece growth (NEw) and fetal growth (NEf)]. However, there are...