Summary of the structure and function of eukaryotic cells. This page covers cell envelope and extensions of eukaryotes.
Tremblay, Sylvie. "Eukaryotic Cell: Definition, Structure & Function (With Analogy & Diagram)"sciencing.com, https://www.sciencing.com/eukaryotic-cell-definition-structure-function-with-analogy-diagram-13717298/. 24 June 2019. APA Tremblay, Sylvie. (2019, June 24). Eukaryotic Cell: Definition, S...
IRES efficacy in varying cell and sequence contexts. a Luminescence in the supernatant of HEK293 cells 24 h after transfection with circRNA containing a panel of viral 5′ UTR IRES sequences in GLuc (left axis, dark purple with black outline) and FLuc (right axis, light purple with gray...
The single vector approach expresses all component proteins from a single expression construct, which facilitates easy establishment of expression strains, and ensures expression of component proteins in the same host cell. When the component proteins are expressed as a single chain protein, it guara...
et al. A global genetic interaction network maps a wiring diagram of cellular function. Science 353, https://doi.org/10.1126/science.aaf1420 (2016). Sheu, Y. J., Barral, Y. & Snyder, M. Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae. Mol...
Bacterial DNA was isolated from cell pellets of overnight cultures using the DNeasy 96 Blood & Tissue Kit (Qiagen) according to the manufacturers protocol. Amplification followed previously established protocols [14]. Primer details are listed in supporting information (Additional file 1: Table S1)....
(18) and argues against an indispensable role in replication. It seems more likely that these proteins function as primases in a novel DNA repair pathway and/or in specialized DNA amplification systems in specific cell types. The presence of multiple primases in the mimivirus and ASFV is ...
However, it is conceivable that this protein has the effector functions that interfere with the host cell machinery5. In a previous study by our group, it was shown that BipC was present in the secretome of B. pseudomallei laboratory culture and was found to be immunogenic as confirmed by ...
The use of CRISPRa and CRISPRi for assessing Acr activity relative to editing assays affords a few advantages: the assays are quick and can provide quantitative information on Acr activity (rather than a discrete readout of edited / not-edited) on the single-cell level, and thus are ideal ...
Mycovirus and Fungi. Mycelial plugs of Fusarium graminearum strain JS16 with the positive strand RNA virus FgHV255 and Alternaria longipes strain HN28 with the double-stranded RNA virus AlRV156 were incubated on Potato Dextrose Agar in Petri plates overlaid with cell ophanemembranes at 25 ...