Next, RNA-seq reads are mapped using a pseudoalignment algorithm to identify reads that uniquely support deletions. These are then evaluated for evidence of the deletion with respect to gene expression and other samples. We applied the algorithm, named Toblerone, to a cohort of 99 B-ALL ...
Single cell RNA-sequencing enabled by 10x Genomics assays allows for deep analysis of the transcriptome of individual cells, uncovering differences in heterogeneous cell populations that are often masked by traditional bulk methods. With ...