The sections were picked up using a 1:1 mixture of 2% methyl cellulose (Sigma) and 2.3M sucrose. Samples were quenched with 50 mM NH4Cl, blocked in PBS with 1% BSA and immunolabeled with a-hPRXD6 1A11 (SantaCruz, 1:20) IgG1a monoc...
17. Nuclei were isolated by treating cells with 1 ml of a 0.01% Igepal CA-630 (a non-ionic detergent, Sigma) and 1% citric acid solution in water for 5 min. Expelled nuclei from the adherent cells were collected, washed with 5 ml PBS, centrifuged at 800 × g for 5 ...
Cardiomyocytes were grown in DMEM medium containing 10% FBS (GIBCO), γ-interferon (10 U/ml, Sigma), and cardiotrophin (0.2 ng/ml, R&D systems) at 33°C in a humidified atmosphere of 5% CO2. Cultured cardiomyocytes tested positive for expression of MEF2C, Nkx2.5, and desmin by RT-PCR....
The formation of seeds is one of the most important innovations that contribute to the success of flowering plants. By seed formation, the embryo is protected in a desiccated and quiescent state, and, meanwhile, ready to swiftly initiate germination and post-embryonic development when optimal growth...
tri. D. B. J. H. triquetrifolium harvested in Dhrea Ben Jouder, H. tri. Fer. H. triquetrifolium harvested in Fernana, H. tri. F. Dj. H. triquetrifolium harvested in Fondouk Djedid. 1. Bacillus cereus, 2. Enterococcus feacalis, 3. Staphylococcus aureus. 4. Staphylococcus epidermidis...
The substrate was prepared as above except that instead of an olive oil in PVOH emulsion, 1 ml of tributyrin (glyceryl tributyrate, Sigma) was added to the mixture. Wells were cut and loaded as above. The plate was then incubated at 30°C for 3.5 h. RNAi silencing First, DNA ...
Rabbit anti-BBLN Sigma-Aldrich Cat# HPA020725; RRID: AB_1845816 Mouse anti-PCNT Abcam Cat# ab28144; RRID: AB_2160664 Rat anti-Tyrosinated α-tubulin (YL1/2) Thermo Fisher Scientific Cat# MA1-80017; RRID: AB_2210201 Rat anti-KRT8 DSHB Cat# TROMA-I; RRID: AB_531826 Alexa Fluor 568-...
To obtain the fluorescent images of the cell wall, actin cytoskeleton, and nuclear DNA, yeast cells were stained triply with fluorescein isothiocyanate-ConA (Sigma Aldrich, St. Louis, MO, USA), rhodamine-phalloidin (Invitrogen, Carlsbad, CA, USA), and 4′,6-diamidino-2-phenylindole (Sigma Al...
The effectiveness of DNA elimination from RNA samples was checked by PCR using Ready-to-use RED-Taq DNA polymerase mix (Sigma-Aldrich) and primer pairs specific to rhizobial genes, pssA encoding a glucosyltransferase involved in the first step of EPS synthesis (pssAG1f/pssA2r) and pssY ...
5,5-水合柠檬酸三钠 EMPROVE® ESSENTIAL, E 331; CAS Number: 6858-44-2; Synonyms: tri-Sodium citrate 5,5-hydrate at Sigma-Aldrich