ESR1 Antibody,采用蛋白A親和純化方法制成,可與人、小鼠、大鼠、奶牛、馬、兔、綿羊源的ESR1 Antibody產生特異性結合,儲存于0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol中,短期4℃運輸即可,長期應存儲于-20℃,避免反復凍融,ESR1 Antibody表達于細胞膜,分子量為44kDa,應用于細胞生物、細...
As an internal control, rabbit polyclonal anti-TUBB antibody (1:1000; sc-9104, Santa Cruz Biotechnology) was applied. After rinsing with TBST, the membrane was incubated in peroxidase-labeled goat anti-rabbit IgG (1:2000; ab6721, Abcam) or rabbit anti-goat IgG (1:2000; ab6741, Abcam) ...
The sections were incubated with primary antibody anti-VEGFA (1:100- sc-152) overnight at 4 °C in a moist chamber. Next, sections were incubated in a secondary antibody (Goat to rabbit- FITC, Abcam® ab6717) for 1h. Following which, sections were incubated with the membrane marker...
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The blots were immuno-stained using antibodies to ERα (D8H8, Cell Signaling Technology) and phospho- ERα (Ser118) (Signalway Antibody). Immunoblots were repeated twice unless otherwise stated. Transcriptional reporter activity of WT and mutant ESR1 ESR1 WT and mutant cells were transfected with...
Antibodies for western blot and/or ChIP analyses were beta-actin (Abcam; Cambridge, UK; ab6278), ERα (Santa Cruz; CA, USA sc-543), non-specific IgG (Abcam; ab46540), MED12 (Bethyl; TX, USA ; ICH-00180), RNAPII (sc-899), Ser2-phosphorylated RNAPII CTD[40], and SMC3 (Abcam...
It is also possible that Jag1 also acts through another Notch receptor to activate Hesr1, and the ActN1-ICD antibody does not recognize the cleavage site of the other Notch receptors (Del Monte et al., 2007). Thus, Jag1/Notch2 signaling might regulate expression of Hesr1, while Jag1/...
basal-like breast cancers, such as higher levels of PD-L1 expression along with constitutive IFNγ signaling activation13, in line with higher immune-infiltration scores6. While the FDA has granted an accelerated approval for atezolizumab, a monoclonal antibody drug targeting PD-L1, plus ...
The estrogen receptor alpha (ESR1) is an important gene transcriptional regulator, known to mediate the effects of estrogen. Canonically, ESR1 is activated by its ligand estrogen. However, the role of unliganded ESR1 in transcriptional regulation has bee