In mammal, the ESCRT-I component VPS37A subunit is required for autophagosome closure, followed by recruitment of the ESCRT-III component CHMP2A31,32. In yeast, the ESCRT-III subunit SNF7 interacts with the scaffold protein Atg17 in a VPS21-dependent manner, resulting in the recruitment of ...
Inhibition of class E proteins and auxiliary proteins (VPS4A, VPS4B, Tsg101, Alix, and Nedd4.1) reduces extracellular virion production, which preliminarily indicates that the ESCRT pathway is involved in TFV release. The respective interactions of TFV VP031L, VP065L, VP093L with Alix, Tsg...
Inhibition of class E proteins and auxiliary proteins (VPS4A, VPS4B, Tsg101, Alix, and Nedd4.1) reduces extracellular virion production, which preliminarily indicates that the ESCRT pathway is involved in TFV release. The respective interactions of TFV VP031L, VP065L, VP093L with Alix, Tsg...
a key checkpoint must suppress ILVs degradation by preventing the fusion of MVEs with lysosomes.1The accumulated nondegradable MVEs use the common secretory machineries for exosome secretion, which is mainly regulated by RAB27.1,15Therefore, exosome biogenesis pathway mainly contains three key steps a...
(prevacuolar compartment) and autophagic pathway. Recent researches have demonstrated that the ESCRT machinery plays an essential role in the vacuolar sorting of ubiquitinated membrane proteins via regulation the biogenesis of multivesicular body (MVB)/PVC (prevacuolar compartment) and autophagosome as well...
of the AAAATPaseVps4/SKD-1. The loss of any of the ESCRT components or Vps4/SKD-1 results in an inhibition of MVB formation and retention of the internalised receptor on thesurfaceof the endosome, which has severe consequences on the duration of the growth factorsignalling pathway[28,29...
the SARS-CoV-2 S protein by inserting a short amino acid sequence (termed an endosomal sorting complex required for transport [ESCRT]- and ALG-2-interacting protein X [ALIX]-binding region or EABR)17 at the C terminus of its cytoplasmic tail to recruit host proteins from the ESCRT pathway...
The multivesicular body (MVB) sorting pathway provides a mechanism for the delivery of cargo destined for degradation to the vacuole or lysosome. The endosomal sorting complex required for transport (ESCRT) is essential for the MVB sorting pathway by driving the cargo sorting to its destination. Ma...
6a,b). These results indicate that ESCRT-III is required for suppression of progerin-induced NM deformation by rapamycin and that autophagy and ESCRT-III act in suppression of progerin-induced NM deformation by rapamycin in the same pathway. Alternatively, upon reduction of progerin levels, ...
Provides the first structure of the active hexameric form of Vps4. Article CAS PubMed Google Scholar Obita, T. et al. Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4. Nature 449, 735–739 (2007). Article CAS PubMed Google Scholar Stuchell-Brereton, M. ...