Cloning and expression of functional reteplase in Escherichia coli top10. Avicenna J Med Biotechnol. 2013;5(3):168-175.Khodabakhsh F, Dehghani Z, Zia MF, Rabbani M, Mir Mohammad Sadeghi H. Cloning and expression of functional reteplase in Escherichia coli top10. Avicenna J Med Biotechnol. ...
We report a method for detecting Escherichia coli using a nematic liquid crystal (LC), 4-cyano-4′-pentylbiphenyl (5CB). Among three E. coli strains tested, TOP10 strain grown on agar plates induces a homeotropic orientation of LCs whereas DH5α and JM109 strains do not. This results ...
Table I. Top 10 over-represented (A) and under-represented (B) peptides in each expression group compared to the average E. coli protein, the average human protein, and to the average group IV protein Peptides were detected using POPPs with probability value of 0.005 and scaling protein le...
The DNA of two E.coli strains was analyzed following transformation- with bacterial cytosine-5-methyltransferase gene M.Msp1 in mammalian transfection vector pcDNA3. Expression of the M.Msp1 was tested by R.Msp1 digestion. The results suggest that the DNA of both strains was fully methylated ...
coli TOP10 (Invitrogen, Carlsbad, CA, USA) unless otherwise stated. Com- mercial Luria–Bertani (LB) was used as the medium for cloning unless otherwise stated. Kanamycin (30 mg ml À 1) was added to the culture medium for antibiotic selection where appropriate. P. aeruginosa In7, a ...
Backbone assignment of the N-terminal 24-kDa fragment of Escherichia coli topoisomerase IV ParE subunit Biomol. NMR Assign, 10 (2016), pp. 135-138 CrossrefGoogle Scholar 38 Y. Shen, F. Delaglio, G. Cornilescu, A. Bax TALOS+: a hybrid method for predicting protein backbone torsion angles...
coli Top10 pcs+ was tested at first, and then beta-lactamase activity in periplasm was examined. Finally Western blot was used to detect the amount of beta-lactamase in both bacterial periplasm and cytoplasm. RESULTS: Antibiotic tests showed that high concentrations of ampicillin inhibited the ...
coli TOP10F' strain used in our laboratory for molecular cloning, but not in any of its precursor strains, including TOP10. Furthermore, bacteria cultured from the mouth of the laboratory worker who performed cDNA RDA were also positive for ILG1.In the course of our studies using cDNA RDA...
In addition to being an important member of the normal intestinal microflora of humans and other mammals, the species Escherichia coli contains many pathotypes that cause a variety of diseases. At least six different pathotypes cause enteric disease, such as diarrhoea or dysentery, and other pathotyp...
coli TOP10 and purified with affinity chromatography using a Ni-NTA column. SDS-PAGE analysis and Western blotting revealed a molecular mass of the mature rEmpA predicted to be 36kDa. The optimal temperature and pH for the purified rEmpA were 37°C and 8.0, respectively. The enzyme was ...